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Literature summary extracted from

  • Baumann, M.J.; Ekloef, J.M.; Michel, G.; Kallas, A.M.; Teeri, T.T.; Czjzek, M.; Brumer, H.
    Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism (2007), Plant Cell, 19, 1947-1963.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.151 isoforms NXG1 and NXG2, comparison with data from the strict endo-transglycolase from Populus tremula x Populus tremuloides Tropaeolum majus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.151 DELTAYNIIG loop deletion variant of isoform NXG1, structurally similar to the strict endo-transglycolase from Populus tremula x Populus tremuloides. Mutant has a greatly increased transglycosylation:hydrolysis ratio Tropaeolum majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 0.08
-
 xyloglucan pH 4.8, isoform NXG1 Tropaeolum majus
3.2.1.151 0.5
-
 xyloglucan pH 4.8, isoform NXG1, transglycosylation of xyloglucan Tropaeolum majus
3.2.1.151 2
-
 xyloglucan pH 4.8, mutant DELTAYNIIG of isoform NXG1, transglycosylation of xyloglucan Tropaeolum majus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Populus tremula x Populus tremuloides Q8GZD5
-
-
3.2.1.151 Tropaeolum majus
-
 isoforms NXG1 and NXG2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Populus tremula x Populus tremuloides ?
-
 ?
2.4.1.207 additional information does not produce any hetero-transglycosylation products with cello-oligosaccharides or lactose Populus tremula x Populus tremuloides ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Tropaeolum majus xyloglucan oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 endo-transglycosylase
-
 Populus tremula x Populus tremuloides
2.4.1.207 XET16-34
-
 Populus tremula x Populus tremuloides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 0.012
-
 xyloglucan pH 4.8, mutant DELTAYNIIG of isoform NXG1, hydrolysis of xyloglucan Tropaeolum majus
3.2.1.151 0.013
-
 xyloglucan pH 4.8, isoform NXG1, transglycosylation of xyloglucan Tropaeolum majus
3.2.1.151 0.015
-
 xyloglucan pH 4.8, mutant DELTAYNIIG of isoform NXG1, transglycosylation of xyloglucan Tropaeolum majus
3.2.1.151 0.08
-
 xyloglucan pH 4.8, isoform NXG1, hydrolysis of xyloglucan Tropaeolum majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 4.8
-
 both isoforms NXG1 and NXG2 Tropaeolum majus