Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schuster, J.; Knill, T.; Reichelt, M.; Gershenzon, J.; Binder, S.
    Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis (2006), Plant Cell, 18, 2664-2679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.045
-
 4-methylsulfanyl-2-oxobutanoate
-
 Arabidopsis thaliana
2.6.1.42 0.93
-
 L-methionine
-
 Arabidopsis thaliana
2.6.1.42 4.86
-
 L-leucine
-
 Arabidopsis thaliana
2.6.1.88 0.045
-
 2-oxo-4-methylthiobutanoate recombinant enzyme, pH and temperature not specified in the publication Arabidopsis thaliana
2.6.1.88 0.93
-
 L-methionine recombinant enzyme, pH and temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.88 cytosol
-
 Arabidopsis thaliana 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 51900
-
 predicted mass Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Arabidopsis thaliana
-
-
-
2.6.1.88 Arabidopsis thaliana Q9LE06 ecotype Col-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 S-protein agarose chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 cotyledon
-
 Arabidopsis thaliana
-
2.6.1.42 leaf
-
 Arabidopsis thaliana
-
2.6.1.42 phloem
-
 Arabidopsis thaliana
-
2.6.1.88 phloem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-homomethionine + 2-oxoglutarate
-
 Arabidopsis thaliana 5-methylthio-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Arabidopsis thaliana 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-methionine + 2-oxoglutarate
-
 Arabidopsis thaliana 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.42 additional information little or no activity is detected with L-isoleucine Arabidopsis thaliana ?
-
 ?
2.6.1.42 additional information no activity is observed with 6-methylthio-2-oxohexanoate, 3-methyl-2-oxopentanoate, or 3-methyl-2-oxobutyrate Arabidopsis thaliana ?
-
 ?
2.6.1.88 2-oxo-4-methylthiobutanoate + an L-amino acid recombinant enzyme shows high activity with 2-oxo-4-methylthiobutanoate (100% activity at 0.1 and 2 mM) Arabidopsis thaliana L-methionine + a 2-oxo acid
-
 r
2.6.1.88 4-methyl-2-oxopentanoate + ? 41% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana L-methionine + a 2-oxo acid
-
 r
2.6.1.88 5-methylthio-2-oxopentanoate + ? 48% activity at 0.1 mM and 53% activity at 2 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana L-methionine + a 2-oxo acid
-
 r
2.6.1.88 L-homomethionine + a 2-oxo acid 37% activity at 1 mM and 65% activity at 5 mM compared to L-methionine Arabidopsis thaliana ?
-
 r
2.6.1.88 L-leucine + a 2-oxo acid 29% activity at 1 mM and 62% activity at 5 mM compared to 2-oxo-4-methylthiobutanoate Arabidopsis thaliana 2-oxo-4-methylthiobutanoate + an L-amino acid
-
 r
2.6.1.88 L-methionine + a 2-oxo acid recombinant enzyme shows high efficiency with L-methionine (100% activity at 1 and 5 mM) Arabidopsis thaliana 2-oxo-4-methylthiobutanoate + an L-amino acid
-
 r
2.6.1.88 additional information the enzyme does not use L-isoleucine (at 1 mM), L-valine (at 1 and 5 mM), 4-methyl-2-oxopentanoate (at 0.1 mM), 6-methylthio-2-oxohexanoate, 3-methyl-2-oxopentanoate, and 3-methyl-2-oxobutyrate as substrates (at 0.1 mM each) Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 At5g17310
-
 Arabidopsis thaliana
2.6.1.42 BCAT4
-
 Arabidopsis thaliana
2.6.1.42 branched-chain aminotransferase4
-
 Arabidopsis thaliana
2.6.1.88 BCAT4
-
 Arabidopsis thaliana
2.6.1.88 branched-chain aminotransferase4
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.6.1.88 Arabidopsis thaliana branched-chain aminotransferase4 transcription is induced by wounding. Branched-chain aminotransferase4 mRNA accumulation is light dependent up

General Information

EC Number General Information Comment Organism
2.6.1.88 metabolism branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis Arabidopsis thaliana