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Literature summary extracted from

  • Almeida, M.; Filipe, S.; Humanes, M.; Maia, M.F.; Melo, R.; Severino, N.; da Silva, J.A.; Frausto da Silva, J.J.; Wever, R.
    Vanadium haloperoxidases from brown algae of the Laminariaceae family (2001), Phytochemistry, 57, 633-642.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.B6 by extensive dialysis with citrate-phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria hyperborea
1.11.1.B6 by extensive dialysis with citrate–phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria ochroleuca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.B6 0.12
-
 H2O2 pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 0.137
-
 H2O2 pH 6.7, isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 0.166
-
 H2O2 pH 6.7, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 0.173
-
 H2O2 pH 6.7, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 0.217
-
 H2O2 pH 6.1, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 0.243
-
 H2O2 pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 0.273
-
 H2O2 pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 0.275
-
 H2O2 pH 6.1, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 0.285
-
 H2O2 pH 5.5, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 0.333
-
 H2O2 pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 0.334
-
 H2O2 pH 5.5, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 0.376
-
 H2O2 pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 1.3
-
 I- pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 1.9
-
 I- pH 5.5, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 1.9
-
 I- pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 2.3
-
 I- pH 5.5, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 2.7
-
 I- pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 3.4
-
 I- pH 6.1, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 3.4
-
 I- pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 3.5
-
 I- pH 6.7, isoenzyme Lh1 Laminaria hyperborea
1.11.1.B6 3.7
-
 I- pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
1.11.1.B6 3.8
-
 I- pH 6.1, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 3.8
-
 I- pH 6.7, isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 4.3
-
 I- pH 6.7, isoenzyme Ls2 Laminaria ochroleuca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B6 Vanadium enzyme contains vanadium Laminaria ochroleuca
1.11.1.B6 Vanadium the enzyme contains vanadium Laminaria hyperborea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.B6 65000
-
 x * 65000, SDS-PAGE Laminaria ochroleuca
1.11.1.B6 67000
-
 x * 67000, SDS-PAGE Laminaria hyperborea
1.11.1.B6 67000
-
 x * 67000, SDS-PAGE Laminaria ochroleuca

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.B6 1-propanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 1-propanol 50% v/v, 30 day incubation, more than 95% loss of activity of isoenzyme Ls1, about 75% loss of activity of isoenzyme Ls2 Laminaria ochroleuca
1.11.1.B6 Acetone 50% v/v, 30 day incubation, about 80% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 Acetone 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
1.11.1.B6 Ethanol 50% v/v, 30 day incubation, about 90% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 Ethanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
1.11.1.B6 Methanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1.11.1.B6 Methanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B6 Laminaria hyperborea
-
-
-
1.11.1.B6 Laminaria ochroleuca
-
-
-
1.11.1.18 Laminaria hyperborea
-
-
-
1.11.1.18 Saccharina latissima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B6 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone. Isoenzyme Lh2 shows 5% of the activity with iodide + H2O2, isoenzyme Lh1 is inactive Laminaria hyperborea ?
-
 ?
1.11.1.B6 I- + H2O2
-
 Laminaria hyperborea triiodide + ?
-
 ?
1.11.1.B6 I- + H2O2
-
 Laminaria ochroleuca triiodide + ?
-
 ?
1.11.1.B6 additional information no monochlorodimedonebromination activity Laminaria ochroleuca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.B6 ? x * 67000, SDS-PAGE Laminaria hyperborea
1.11.1.B6 ? x * 67000, SDS-PAGE Laminaria ochroleuca
1.11.1.B6 ? x * 65000, SDS-PAGE Laminaria ochroleuca

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 BrPO
-
 Saccharina latissima
1.11.1.18 BrPO
-
 Laminaria hyperborea
1.11.1.18 vanadium bromoperoxidase
-
 Saccharina latissima
1.11.1.18 vanadium bromoperoxidase
-
 Laminaria hyperborea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.B6 40
-
 isoenzyme Ls1 Laminaria ochroleuca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.B6 30 50 stable Laminaria hyperborea
1.11.1.B6 30 50 stable Laminaria ochroleuca