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Literature summary extracted from

  • Chen, L.S.; Qi, Y.P.
    Dithiothreitol decreases in vitro activity of ADP-glucose pyrophosphorylase from leaves of apple (Malus domestica Borkh.) and many other plant species (2007), Phytochem. Anal., 18, 300-305.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate in leaves 2-3fold, DTT decreases the activation Solanum lycopersicum
2.7.7.27 3-phosphoglycerate in leaves 2-3fold, DTT highly decreases the activation Glycine max
2.7.7.27 3-phosphoglycerate in leaves 2fold, DTT abolishes the activation at 5 mM Triticum aestivum
2.7.7.27 3-phosphoglycerate in leaves 2fold, DTT abolishes the activation at 5 mM Arabidopsis thaliana
2.7.7.27 3-phosphoglycerate in leaves 2fold, DTT decreases the activation Hordeum vulgare
2.7.7.27 3-phosphoglycerate in leaves 30fold, DTT abolishes the activation at 1-5 mM Spinacia oleracea
2.7.7.27 3-phosphoglycerate in leaves and tubers 1.4-3.8fold, DTT decreases the activation, overview Solanum tuberosum
2.7.7.27 3-phosphoglycerate slight activation, DTT abolishes the activation Sorghum sp.
2.7.7.27 dithiothreitol in absence of 3-phosphoglycerate, increase of acitivity for enzyme from tuber, no effect on enzyme from leaf Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 dithiothreitol in presence of 3-phophoglycerate, decrease of acitivity Arabidopsis thaliana
2.7.7.27 dithiothreitol inhibition is prevented by pre- or by coincubation with 3-phophoglycerate, but incubation of already inhibited enzyme with 3-phophoglycerate may not reverse the inhibition Malus domestica
2.7.7.27 dithiothreitol in presence of 3-phophoglycerate, decrease of acitivity for enzyme from tuber Solanum tuberosum
2.7.7.27 DTT 3-phosphoglycerate protects completely when added before the inhibition, but does not affect the enzyme activity after inhibition by DTT takes place Malus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+
-
Triticum aestivum
2.7.7.27 Mg2+
-
Hordeum vulgare
2.7.7.27 Mg2+
-
Spinacia oleracea
2.7.7.27 Mg2+
-
Solanum tuberosum
2.7.7.27 Mg2+
-
Glycine max
2.7.7.27 Mg2+
-
Arabidopsis thaliana
2.7.7.27 Mg2+
-
Solanum lycopersicum
2.7.7.27 Mg2+
-
Sorghum sp.
2.7.7.27 Mg2+
-
Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Triticum aestivum
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Hordeum vulgare
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Spinacia oleracea
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Solanum tuberosum
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Glycine max
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Arabidopsis thaliana
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Solanum lycopersicum
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Sorghum sp.
-
ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Malus domestica
-
ADP-D-glucose + diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Arabidopsis thaliana
-
-
-
2.7.7.27 Glycine max
-
-
-
2.7.7.27 Hordeum vulgare
-
-
-
2.7.7.27 Malus domestica
-
-
-
2.7.7.27 Solanum lycopersicum
-
-
-
2.7.7.27 Solanum tuberosum
-
-
-
2.7.7.27 Sorghum sp.
-
-
-
2.7.7.27 Spinacia oleracea
-
-
-
2.7.7.27 Triticum aestivum
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.7.27 inhibition of enzyme by dithiothreitol is prevented by pre- or by coincubation with 3-phophoglycerate, but incubation of already inhibited enzyme with 3-phophoglycerate may not reverse the inhibition Solanum tuberosum
2.7.7.27 inhibition of enzyme by dithiothreitol is prevented by pre- or by coincubation with 3-phophoglycerate, but incubation of already inhibited enzyme with 3-phophoglycerate may not reverse the inhibition Arabidopsis thaliana
2.7.7.27 inhibition of enzyme by dithiothreitol is prevented by pre- or by coincubation with 3-phophoglycerate, but incubation of already inhibited enzyme with 3-phophoglycerate may not reverse the inhibition Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 leaf
-
Triticum aestivum
-
2.7.7.27 leaf
-
Hordeum vulgare
-
2.7.7.27 leaf
-
Spinacia oleracea
-
2.7.7.27 leaf
-
Solanum tuberosum
-
2.7.7.27 leaf
-
Glycine max
-
2.7.7.27 leaf
-
Arabidopsis thaliana
-
2.7.7.27 leaf
-
Solanum lycopersicum
-
2.7.7.27 leaf
-
Sorghum sp.
-
2.7.7.27 leaf
-
Malus domestica
-
2.7.7.27 tuber
-
Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Triticum aestivum ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Hordeum vulgare ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Spinacia oleracea ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Solanum tuberosum ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Glycine max ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Arabidopsis thaliana ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Solanum lycopersicum ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Sorghum sp. ADP-D-glucose + diphosphate
-
r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
Malus domestica ADP-D-glucose + diphosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 ADP-glucose pyrophosphorylase
-
Triticum aestivum
2.7.7.27 ADP-glucose pyrophosphorylase
-
Hordeum vulgare
2.7.7.27 ADP-glucose pyrophosphorylase
-
Spinacia oleracea
2.7.7.27 ADP-glucose pyrophosphorylase
-
Solanum tuberosum
2.7.7.27 ADP-glucose pyrophosphorylase
-
Glycine max
2.7.7.27 ADP-glucose pyrophosphorylase
-
Arabidopsis thaliana
2.7.7.27 ADP-glucose pyrophosphorylase
-
Solanum lycopersicum
2.7.7.27 ADP-glucose pyrophosphorylase
-
Sorghum sp.
2.7.7.27 ADP-glucose pyrophosphorylase
-
Malus domestica
2.7.7.27 AGPase
-
Triticum aestivum
2.7.7.27 AGPase
-
Hordeum vulgare
2.7.7.27 AGPase
-
Spinacia oleracea
2.7.7.27 AGPase
-
Solanum tuberosum
2.7.7.27 AGPase
-
Glycine max
2.7.7.27 AGPase
-
Arabidopsis thaliana
2.7.7.27 AGPase
-
Solanum lycopersicum
2.7.7.27 AGPase
-
Sorghum sp.
2.7.7.27 AGPase
-
Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.27 37
-
assay at Triticum aestivum
2.7.7.27 37
-
assay at Hordeum vulgare
2.7.7.27 37
-
assay at Spinacia oleracea
2.7.7.27 37
-
assay at Solanum tuberosum
2.7.7.27 37
-
assay at Glycine max
2.7.7.27 37
-
assay at Arabidopsis thaliana
2.7.7.27 37
-
assay at Solanum lycopersicum
2.7.7.27 37
-
assay at Sorghum sp.
2.7.7.27 37
-
assay at Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 8
-
assay at Triticum aestivum
2.7.7.27 8
-
assay at Hordeum vulgare
2.7.7.27 8
-
assay at Spinacia oleracea
2.7.7.27 8
-
assay at Solanum tuberosum
2.7.7.27 8
-
assay at Glycine max
2.7.7.27 8
-
assay at Arabidopsis thaliana
2.7.7.27 8
-
assay at Solanum lycopersicum
2.7.7.27 8
-
assay at Sorghum sp.
2.7.7.27 8
-
assay at Malus domestica