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Literature summary extracted from

  • Suzuki, Y.; Doi, M.; Shioi, Y.
    Two enzymatic reaction pathways in the formation of pyropheophorbide a (2002), Photosynth. Res., 74, 225-233.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.82 EDTA 1 mM, 22% activation, Phedase type 1 Raphanus sativus
3.1.1.82 EDTA 1 mM, 9% activation, Phedase type 2 Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.82 methanol 1 M, 20% inhibition, Phedase type 1; 1 M, 20% inhibition, Phedase type 2 Raphanus sativus
3.1.1.82 N-ethylmaleimide 1 mM, 11% inhibition, Phedase type 1 Raphanus sativus
3.1.1.82 phenylmethylsulfonic fluoride 1 mM, 26.4% inhibition, Phedase type 2; 1 mM, 61% inhibition, Phedase type 1 Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.82 0.0141
-
 pheophorbide a 30°C, pH 7.0, Phedase type 1 Raphanus sativus
3.1.1.82 0.0151
-
 pheophorbide a 30°C, pH 7.0, Phedase type 2 Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.82 57000
-
 2 * 57000, Phedase type 1, SDS-PAGE Raphanus sativus
3.1.1.82 113000
-
 Phedase type 1, gel filtration Raphanus sativus
3.1.1.82 113000
-
 Phedase type 2, gel filtration Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.82 pheophorbide a + H2O Raphanus sativus Phedase type 1 is senescence-induced pyropheophorbide a + methanol + CO2
-
 ?
3.1.1.82 pheophorbide a + H2O Raphanus sativus Phedase type 2 is constitutively produced pyropheophorbide a + methanol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.82 Arabidopsis thaliana
-
 low activity
-
3.1.1.82 Brassica napus
-
-
-
3.1.1.82 Brassica oleracea
-
-
-
3.1.1.82 Brassica rapa subsp. chinensis
-
-
-
3.1.1.82 Brassica rapa subsp. oleifera
-
-
-
3.1.1.82 Brassica rapa subsp. pekinensis
-
 low activity
-
3.1.1.82 Chenopodium album
-
-
-
3.1.1.82 Hypolepis punctata
-
-
-
3.1.1.82 Nasturtium officinale
-
-
-
3.1.1.82 no activity in Camellia sinensis
-
-
-
3.1.1.82 no activity in Chlamydomonas reinhardtii
-
-
-
3.1.1.82 no activity in Chrysanthemum coronarium
-
-
-
3.1.1.82 no activity in Corchorus olitorius
-
-
-
3.1.1.82 no activity in Equisetum arvense
-
-
-
3.1.1.82 no activity in Ginkgo biloba
-
-
-
3.1.1.82 no activity in Hordeum vulgare
-
-
-
3.1.1.82 no activity in Lepisorus thunbergianus
-
-
-
3.1.1.82 no activity in Perilla frutescens
-
-
-
3.1.1.82 no activity in Petroselinum sativum
-
-
-
3.1.1.82 no activity in Pisum sativum
-
-
-
3.1.1.82 no activity in Rumex acetosa
-
-
-
3.1.1.82 no activity in Spinacia oleracea
-
-
-
3.1.1.82 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.82 Phedase type 1 Raphanus sativus
3.1.1.82 Phedase type 2 Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.82 cotyledon
-
 Raphanus sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.82 pheophorbide a + H2O Phedase type 1 is senescence-induced Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?
3.1.1.82 pheophorbide a + H2O Phedase type 2 is constitutively produced Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?
3.1.1.82 pheophorbide a + H2O the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 1 Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?
3.1.1.82 pheophorbide a + H2O the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 2 Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.82 dimer
-
 Raphanus sativus
3.1.1.82 dimer 2 * 57000, Phedase type 1, SDS-PAGE Raphanus sativus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.82 phedase
-
 Arabidopsis thaliana
3.1.1.82 phedase
-
 Brassica napus
3.1.1.82 phedase
-
 Raphanus sativus
3.1.1.82 phedase
-
 Brassica oleracea
3.1.1.82 phedase
-
 Brassica rapa subsp. oleifera
3.1.1.82 phedase
-
 Brassica rapa subsp. pekinensis
3.1.1.82 phedase
-
 Nasturtium officinale
3.1.1.82 phedase
-
 Chenopodium album
3.1.1.82 phedase
-
 Brassica rapa subsp. chinensis
3.1.1.82 phedase
-
 Hypolepis punctata
3.1.1.82 Phedase type 1
-
 Raphanus sativus
3.1.1.82 Phedase type 2
-
 Raphanus sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.82 68
-
 50% loss of activity at 67.5°C, Phedase type 1 Raphanus sativus
3.1.1.82 68
-
 50% loss of activity at 67.5°C, Phedase type 2 Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.82 6.5
-
 Phedase type 2 Raphanus sativus
3.1.1.82 7 7.5 Phedase type 1 Raphanus sativus