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Literature summary extracted from

  • Thomas, C.B.; Gumport, R.I.
    Dimerization of the bacterial RsrI N6-adenine DNA methyltransferase (2006), Nucleic Acids Res., 34, 806-815.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.72 analysis the dimer stabilizes and enhances site-specific DNA-binding by the MTase, because the DNA-binding site may require an interfacial structure formed by both monomers, role of Type II MTases as defense systems against phage Cereibacter sphaeroides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 mutant expressed in Escherichia coli BL21(DE3) Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 S124D reduced solubility of the protein relative to that of the wild-type enzyme, fails to crystallize under the same conditions used to crystallize wild-type enzyme, is active and shows a non-linear dependence of activity on enzyme concentration similar as wild-type Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 0.00000000000001
-
 DNA adenine His-tagged wild-type Cereibacter sphaeroides
2.1.1.72 0.00000000000002
-
 DNA adenine mutant S124D Cereibacter sphaeroides
2.1.1.72 0.00086
-
 S-adenosyl-L-methionine mutant S124D Cereibacter sphaeroides
2.1.1.72 0.0027
-
 S-adenosyl-L-methionine His-tagged wild-type Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 30000
-
 gel filtration, mutant S124D Cereibacter sphaeroides
2.1.1.72 74400
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 mutant purified by nickel-ion metal-affinity chromatography, more than 95% pure Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
2.1.1.72 -80°C Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 dimer gel filtration Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 M.RsrI
-
 Cereibacter sphaeroides
2.1.1.72 RsrI N6-adenine DNA methyltransferase
-
 Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.72 0.000167
-
 DNA adenine mutant S124D Cereibacter sphaeroides
2.1.1.72 0.0035
-
 DNA adenine His-tagged wild-type Cereibacter sphaeroides