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Literature summary extracted from

  • Wang, H.; Brautigan, D.L.
    Peptide microarray analysis of substrate specificity of the transmembrane Ser/Thr kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase (2006), Mol. Cell. Proteomics, 5, 2124-2130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.19 expression of c-Myc-/His6-tagged full-length KPI-2 and minimal kinase fold, residues 137-407 plus an additional C-terminal segment in HEK-293 cells and in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 additional information KPI-2 is inhibited in living cells by addition of nerve growth factor or serum Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.19 membrane KPI-2 is a transmembrane protein Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.19 ATP + phosphorylase b Homo sapiens
-
 ADP + phosphorylated phosphorylase b
-
 ?
2.7.11.19 additional information Homo sapiens hormonal regulation of KPI-2, kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 recombinant c-Myc-/His6-tagged full-length KPI-2 from Sf9 cells by nickel affinity and ion exchange chromatography, and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b
-
 Homo sapiens ADP + phosphorylated phosphorylase b
-
 ?
2.7.11.19 ATP + phosphorylase b KPI-2 reacts with Ser residues either preceded by or followed by Pro residues, does not strictly require an adjacent Pro residue Homo sapiens ADP + phosphorylated phosphorylase b
-
 ?
2.7.11.19 additional information hormonal regulation of KPI-2, kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.19 kinase kinase/phosphatase/inhibitor-2
-
 Homo sapiens
2.7.11.19 KPI-2 kinase
-
 Homo sapiens
2.7.11.19 transmembrane Ser/Thr kinase KPI-2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 ATP
-
 Homo sapiens