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Literature summary extracted from

  • Klomklang, W.; Tani, A.; Kimbara, K.; Mamoto, R.; Ueda, T.; Shimao, M.; Kawai, F.
    Biochemical and molecular characterization of a periplasmic hydrolase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3 (2005), Microbiology, 151, 1255-1262.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.7.1.7 molecular biology exhibits 63% identity with OPH of Pseudomonas sp. strain VM15C and 29-32% identity with the polyhydroxybutyrate depolymerases from Mesorhizobium loti, Rhizobium sp. and Sinorhizobium meliloti Sphingomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.7 expression in Escherichia coli under the control of the lac promoter Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.7 Hg2+ appreciably inhibits Sphingomonas sp.
3.7.1.7 additional information not inhibited by chelating agents, thiol agents and inhibitors of the respiratory chain Sphingomonas sp.
3.7.1.7 PMSF 1 mM strongly inhibits Sphingomonas sp.
3.7.1.7 Zn2+ appreciably inhibits Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.7 0.2
-
 Oxidized polyvinyl alcohol
-
 Sphingomonas sp.
3.7.1.7 0.3
-
 p-nitrophenyl acetate
-
 Sphingomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.7.1.7 periplasm
-
 Sphingomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.7 Fe3+ slightly activates Sphingomonas sp.
3.7.1.7 Mg2+ slightly activates Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.7 35000
-
 2 * 35000, SDS-PAGE Sphingomonas sp.
3.7.1.7 70000
-
 gel filtration Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.7 Sphingomonas sp.
-
-
-
3.7.1.7 Sphingomonas sp. 113P3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.7 to homogeneity, 5-9fold, gel filtration Sphingomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.7 0.1
-
 with oxidized polyvinyl alcohol as substrate Sphingomonas sp.
3.7.1.7 2.6
-
 with p-nitrophenyl acetate as substrate Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.7 additional information no activity on monoketones, diketones and esters Sphingomonas sp. ?
-
 ?
3.7.1.7 additional information no activity on monoketones, diketones and esters Sphingomonas sp. 113P3 ?
-
 ?
3.7.1.7 oxidized polyvinyl alcohol + H2O
-
 Sphingomonas sp. ?
-
 ?
3.7.1.7 oxidized polyvinyl alcohol + H2O
-
 Sphingomonas sp. 113P3 ?
-
 ?
3.7.1.7 p-nitrophenyl acetate + H2O
-
 Sphingomonas sp. ?
-
 ?
3.7.1.7 p-nitrophenyl acetate + H2O
-
 Sphingomonas sp. 113P3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.7 homodimer 2 * 35000, SDS-PAGE Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.7.1.7 OPH
-
 Sphingomonas sp.
3.7.1.7 oxidized polyvinyl alcohol hydrolase
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.7 37
-
-
 Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.7 30
-
 stable for 30 min, 50 mM Tris/HCl buffer, pH 8.0 Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.7 8
-
-
 Sphingomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.7 6.5 8.5 50 mM sodium phosphate or Tris/HCl buffer Sphingomonas sp.