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Literature summary extracted from

  • Luo, S.; Liu, D.Q.; Liu, H.; Zhou, N.Y.
    Site-directed mutagenesis of gentisate 1,2-dioxygenases from Klebsiella pneumoniae M5a1 and Ralstonia sp. strain U2 (2006), Microbiol. Res., 161, 138-144.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expressed in Escherichia coli Rosetta cells Klebsiella pneumoniae
1.13.11.4 expressed in Escherichia coli Rosetta cells Ralstonia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.4 H108D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H108D complete loss of activity Ralstonia sp.
1.13.11.4 H110D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H110D complete loss of activity Ralstonia sp.
1.13.11.4 H118D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H118D complete loss of activity Ralstonia sp.
1.13.11.4 H120D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H120D complete loss of activity Ralstonia sp.
1.13.11.4 H149D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H149D complete loss of activity Ralstonia sp.
1.13.11.4 H151D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H151D complete loss of activity Ralstonia sp.
1.13.11.4 H159D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H159D complete loss of activity Ralstonia sp.
1.13.11.4 H161D complete loss of activity Klebsiella pneumoniae
1.13.11.4 H161D complete loss of activity Ralstonia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 35000
-
 SDS-PAGE Klebsiella pneumoniae
1.13.11.4 35000
-
 SDS-PAGE Ralstonia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Klebsiella pneumoniae
-
-
-
1.13.11.4 Klebsiella pneumoniae M5a1
-
-
-
1.13.11.4 Ralstonia sp.
-
-
-
1.13.11.4 Ralstonia sp. U2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Klebsiella pneumoniae maleylpyruvate
-
 ir
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Ralstonia sp. maleylpyruvate
-
 ir
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Ralstonia sp. U2 maleylpyruvate
-
 ir
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Klebsiella pneumoniae M5a1 maleylpyruvate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 GDO
-
 Klebsiella pneumoniae
1.13.11.4 GDO
-
 Ralstonia sp.