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Literature summary extracted from

  • Scandurra, G.M.; Ryan, A.A.; Pinto, R.; Britton, W.J.; Triccas, J.A.
    Contribution of L-alanine dehydrogenase to in vivo persistence and protective efficacy of the BCG vaccine (2006), Microbiol. Immunol., 50, 805-810.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.1.1 medicine L-alanine catabolism can be conferred on Mycobacterium bovis BCG by introduction of the gene encoding Ald of Mycobacterium tuberculosis, restoration of Ald activity does not change the in vivo growth of BCG in macrophages or mice, and protection against aerosol Mycobacterium tuberculosis infection is not altered by addition of the gene encoding Ald to the BCG vaccine Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 into vector pJEX58, resulting plasmid pGS3 expressed in Mycobacterium bovis BCG Pasteur Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 40000
-
 immunoblotting, detectable in sonicates of Mycobacterium bovis BCG harbouring the pGS3 vector Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Mycobacterium tuberculosis
-
-
-
1.4.1.1 no activity in Mycobacterium bovis BCG
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ Mycobacterium tuberculosis displays less Ald activity than the complemented Mycobacterium bovis BCG strain Mycobacterium tuberculosis pyruvate + NH3 + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALD
-
 Mycobacterium tuberculosis
1.4.1.1 L-alanine dehydrogenase
-
 Mycobacterium tuberculosis
1.4.1.1 NAD+ dependent amino acid dehydrogenase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NADH
-
 Mycobacterium tuberculosis