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Literature summary extracted from

  • Soerensen, B.M.; Furukawa-Stoffer, T.L.; Marshall, K.S.; Page, E.K.; Mir, Z.; Forster, R.J.; Weselake, R.J.
    Storage lipid accumulation and acyltransferase action in developing flaxseed (2005), Lipids, 40, 1043-1049.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Linum usitatissimum
-
-
2.3.1.51 microsome
-
 Linum usitatissimum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+
-
 Linum usitatissimum
2.3.1.51 Mg2+
-
 Linum usitatissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA Linum usitatissimum
-
 triacylglycerol + CoA
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Linum usitatissimum the enzyme catalyzes the second acylations in the glycerol-3-phosphate pathway CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Linum usitatissimum
-
 cvs. AC Emerson and Vimy
-
2.3.1.51 Linum usitatissimum
-
 cvs. AC Emerson and Vimy
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 native enzyme partially by microsome preparation Linum usitatissimum
2.3.1.51 native enzyme partially by microsome preparation Linum usitatissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 embryo
-
 Linum usitatissimum
-
2.3.1.20 seed developmenting, maximal activity at 8-14 days after flowering during rapid lipid accumulation, overview Linum usitatissimum
-
2.3.1.51 embryo
-
 Linum usitatissimum
-
2.3.1.51 seed developmenting, maximal activity at 8-14 days after flowering during rapid lipid accumulation, overview Linum usitatissimum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 additional information
-
 lipid content and fatty acid composition of triacylglycerols during seed development, overview Linum usitatissimum
2.3.1.51 additional information
-
 lipid content and fatty acid composition of triacylglycerols during seed development, overview Linum usitatissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Linum usitatissimum triacylglycerol + CoA
-
 ?
2.3.1.20 additional information acyl-CoA specificity, overview Linum usitatissimum ?
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Linum usitatissimum CoA + triolein
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate the enzyme catalyzes the second acylations in the glycerol-3-phosphate pathway Linum usitatissimum CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate the preferred substrate of LPAAT is 18:2-CoA Linum usitatissimum CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information developmental and substrate specificity, overview Linum usitatissimum ?
-
 ?
2.3.1.51 oleoyl-CoA + lysophosphatidic acid
-
 Linum usitatissimum CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT
-
 Linum usitatissimum
2.3.1.20 diacylglycerol acyltransferase
-
 Linum usitatissimum
2.3.1.51 LPAAT
-
 Linum usitatissimum
2.3.1.51 lysophosphatidic acid acyltransferase
-
 Linum usitatissimum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 30
-
 assay at Linum usitatissimum
2.3.1.51 30
-
 assay at Linum usitatissimum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.4
-
 assay at Linum usitatissimum
2.3.1.51 7.4
-
 assay at Linum usitatissimum