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Literature summary extracted from

  • Walker, J.R.; Poulter, C.D.
    Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthase (2005), J. Org. Chem., 70, 9955-9959.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 (2R,3R)-2,3,4-trihydroxybutyl dihydrogen phosphate IC50: 0.310 mM Escherichia coli
1.1.1.267 (2R,3R)-4-amino-2,3-dihydroxybutyl dihydrogen phosphate very weak inhibitor, IC50: 5 mM, above Escherichia coli
1.1.1.267 (2R,3S)-2,3-dihydroxy-4-(hydroxyamino)-4-oxobutyl dihydrogen phosphate very weak inhibitor, IC50: 5 mM, above Escherichia coli
1.1.1.267 (2R,3S)-4-amino-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate IC50: 0.253 mM Escherichia coli
1.1.1.267 (2S,3R)-2,3-dihydroxy-4-phosphonooxybutyric acid IC50: 0.551 mM Escherichia coli
1.1.1.267 (2S,3R)-methyl 2,3-dihydroxy-4-phosphonooxybutyrate IC50: 1.024 mM Escherichia coli
1.1.1.267 fosmidomycin a phosphonic metabolite from Streptomyces rubellomurinus Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.081
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant His-tagged enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli involved in the biosynthesis of isopentenylphosphate 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ involved in the biosynthesis of isopentenylphosphate Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 MEP synthase
-
 Escherichia coli
1.1.1.267 methylerythritol phosphate synthase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 33
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant His-tagged enzyme Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 0.0000021
-
 fosmidomycin
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 0.253
-
 IC50: 0.253 mM Escherichia coli (2R,3S)-4-amino-2,3-dihydroxy-4-oxobutyl dihydrogen phosphate
1.1.1.267 0.31
-
 IC50: 0.310 mM Escherichia coli (2R,3R)-2,3,4-trihydroxybutyl dihydrogen phosphate
1.1.1.267 0.551
-
 IC50: 0.551 mM Escherichia coli (2S,3R)-2,3-dihydroxy-4-phosphonooxybutyric acid
1.1.1.267 1.024
-
 IC50: 1.024 mM Escherichia coli (2S,3R)-methyl 2,3-dihydroxy-4-phosphonooxybutyrate
1.1.1.267 5
-
 very weak inhibitor, IC50: 5 mM, above Escherichia coli (2R,3S)-2,3-dihydroxy-4-(hydroxyamino)-4-oxobutyl dihydrogen phosphate
1.1.1.267 5
-
 very weak inhibitor, IC50: 5 mM, above Escherichia coli (2R,3R)-4-amino-2,3-dihydroxybutyl dihydrogen phosphate