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Literature summary extracted from

  • Ishii, H.; Shibuya, K.; Ohta, Y.; Mukai, H.; Uchino, S.; Takata, N.; Rose, J.A.; Kawato, S.
    Enhancement of nitric oxide production by association of nitric oxide synthase with N-methyl-D-aspartate receptors via postsynaptic density 95 in genetically engineered Chinese hamster ovary cells: real-time fluorescence imaging using nitric oxide sensiti (2006), J. Neurochem., 96, 1531-1539.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 stable, functional co-expression of enzyme and NMDA receptors in CHO1 cell plasma membranes, predominantly, and in the cytosol of CHO1(–) cells lacking postsynaptic density 95 proteins, PSD-95, expression Cricetulus griseus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 additional information enhancement of nitric oxide production by association of neuronal nitric oxide synthase with N-methyl-D-aspartate receptors via postsynaptic density 95 proteins in genetically engineered Chinese hamster ovary cells, method development and quantitative analysis, overview Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 membrane
-
 Cricetulus griseus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Cricetulus griseus
-
 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information Cricetulus griseus postsynaptic density 95 proteins mediate the complex formation of neuronal nitric oxide synthase and N-methyl-D-aspartate receptors ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 CHO cell
-
 Cricetulus griseus
-
1.14.13.39 neuron
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Cricetulus griseus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information postsynaptic density 95 proteins mediate the complex formation of neuronal nitric oxide synthase and N-methyl-D-aspartate receptors Cricetulus griseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 neuronal nitric oxide synthase
-
 Cricetulus griseus
1.14.13.39 nNOS
-
 Cricetulus griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
 assay at Cricetulus griseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 NADPH
-
 Cricetulus griseus