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Literature summary extracted from

  • Yu, H.; Shi, Y.; Luo, H.; Tian, Z.; Zhu, Y.; Shen, Z.
    An over expression and high efficient mutation system of a cobalt-containing nitrile hydratase (2006), J. Mol. Catal. B, 43, 80-85.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 Nocardia sp.(strain YS-2002) NHase gene with modified start codon in alpha subunit is overexpressed in Escherichia coli, strain BL21(DE3) Nocardia sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.84 S122A alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
4.2.1.84 S122C alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
4.2.1.84 S122D alpha subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.
4.2.1.84 W47E beta subunit, site-directed mutagenesis of the recombinant NHase with modified start codon Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 Acrylamide mutant enzyme S122C shows higher tolerance under increasing acrylamide levels than recombinant NHase with modified start codon only Nocardia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 82
-
 acrylonitrile recombinant NHase with modified start codon only Nocardia sp.
4.2.1.84 86
-
 acrylonitrile wild type NHase Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Nocardia sp. Q8GJG6 alpha-subunit
-
4.2.1.84 Nocardia sp. Q8GJG7 beta-subunit
-
4.2.1.84 Nocardia sp. YS-2002 Q8GJG6 alpha-subunit
-
4.2.1.84 Nocardia sp. YS-2002 Q8GJG7 beta-subunit
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
 no activity in S122A and S122D mutant proteins Nocardia sp.
4.2.1.84 94.3
-
 W47E strain 1 Nocardia sp.
4.2.1.84 96.8
-
 W47E strain 2 Nocardia sp.
4.2.1.84 128.7
-
 S122C strain 2 Nocardia sp.
4.2.1.84 133.2
-
 S122C strain 1 Nocardia sp.
4.2.1.84 189.2
-
 control strain 1 Nocardia sp.
4.2.1.84 195.1
-
 control strain 2 Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. acrylamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. YS-2002 acrylamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Nocardia sp.
4.2.1.84 nitrile hydratase
-
 Nocardia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 additional information
-
 reaction activation energy of the recombinant NHase with modified start codon is 24.4 +/- 0.5 kJ/mol, this is 37.2% of wild type NHase Nocardia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 5 37 mutant protein W47E shows higher thermo-stability than recombinant NHase with modified start codon only Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 5.5 8.5 wild type NHase Nocardia sp.
4.2.1.84 5.5 7.5 recombinant NHase with modified start codon only Nocardia sp.