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Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities

Gerth, M.L.; Lutz, S.; J. Mol. Biol. 370, 742-751 (2007)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
additional information
construction of libraries of hybrid enzymes by non-homologous recombination of the pyrimidinespecific human thymidine kinase 2 and the broad-specificity dNK from Drosophila melanogaster. Identification of chimeras that phosphorylate nucleoside analogs with higher activity than either parental enzyme, and that possess new activity towards the anti-HIV prodrug 2',3'-didehydro-3'-deoxythymidine
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
Km-values of chimeric enzymes
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2',3'-didehydro-3'-deoxythymidine + ATP
-
675450
Drosophila melanogaster
2',3'-didehydro-3'-deoxythymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2',3'-dideoxycytidine + ATP
-
675450
Drosophila melanogaster
2',3'-dideoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
-
675450
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
675450
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
-
675450
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
675450
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
turnover-numbers of chimeric enzymes
Drosophila melanogaster
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
additional information
construction of libraries of hybrid enzymes by non-homologous recombination of the pyrimidinespecific human thymidine kinase 2 and the broad-specificity dNK from Drosophila melanogaster. Identification of chimeras that phosphorylate nucleoside analogs with higher activity than either parental enzyme, and that possess new activity towards the anti-HIV prodrug 2',3'-didehydro-3'-deoxythymidine
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
Km-values of chimeric enzymes
Drosophila melanogaster
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2',3'-didehydro-3'-deoxythymidine + ATP
-
675450
Drosophila melanogaster
2',3'-didehydro-3'-deoxythymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2',3'-dideoxycytidine + ATP
-
675450
Drosophila melanogaster
2',3'-dideoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
-
675450
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
675450
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
-
675450
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
675450
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
turnover-numbers of chimeric enzymes
Drosophila melanogaster