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Literature summary extracted from

  • Ju, T.; Goldsmith, R.B.; Chai, S.C.; Maroney, M.J.; Pochapsky, S.S.; Pochapsky, T.C.
    One protein, two enzymes revisited: A structural entropy switch interconverts the two isoforms of acireductone dioxygenase (2006), J. Mol. Biol., 363, 823-834.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.53 expressed in Escherichia coli strain BL21(DE3)pLysS Mus musculus
1.13.11.54 expressed in Escherichia coli strain BL21(DE3)pLysS Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.53 H98S mutation results in the formation of a stable soluble protein that while structurally different from ARD-Ni+2 shows a high degree of similarity to the ARD'(Fe) enzyme Mus musculus
1.13.11.54 H98S mutation results in the formation of a stable soluble protein that while structurally different from ARD shows a high degree of similarity to the ARD' enzyme Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.53 Ni2+ Ni2+ can be conservatively replaced by Mn2 +or Co2+, giving rise to ARD activity (CO production) Mus musculus
1.13.11.54 Fe2+ Fe2+ can be replaced by Mg2+, albeit with lower activity Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.53 Mus musculus
-
-
-
1.13.11.54 Mus musculus Q99JT9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.53
-
 Mus musculus
1.13.11.54
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.53 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Mus musculus 4-methylthio-2-ketobutyrate + formate
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Mus musculus 3-(methylthio)propanoate + formate + CO
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.53 monomer x-ray crystallography Mus musculus
1.13.11.54 monomer x-ray crystallography Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.54 ARD'
-
 Mus musculus