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Literature summary extracted from

  • Garcon, A.; Levy, C.; Derrick, J.P.
    Crystal structure of the bifunctional dihydroneopterin aldolase/6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Streptococcus pneumoniae (2006), J. Mol. Biol., 360, 644-653.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.3 expression in Escherichia coli Streptococcus pneumoniae
4.1.2.25 expressed in Escherichia coli BL21 (DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.3 hanging-drop method Streptococcus pneumoniae
4.1.2.25 hanging drop vapour diffusion method with 50 mM MOPS/NaOH (pH 7.0), 17.5% (w/v) methoxy-PEG 2000, 400 mM NaCl, 10 mM MgCl2, 2 mM dithiothreitol, 1 mM EDTA and 5% (v/v) glycerol Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP Streptococcus pneumoniae biosynthesis of folic acid 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.3 Streptococcus pneumoniae
-
-
-
4.1.2.25 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.25 Mono Q 10/10 column chromatography, Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP biosynthesis of folic acid Streptococcus pneumoniae 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?
4.1.2.25 7,8-dihydroneopterin
-
 Streptococcus pneumoniae 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.25 octamer x-ray crystallography Streptococcus pneumoniae
4.1.2.25 tetramer active enzyme in solution Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
-
 Streptococcus pneumoniae
4.1.2.25 DHNA-HPPK DHNA is part of the bifunctional dihydroneopterin aldolase/6 hydroxymethyl-7,8-dihydropterin pyrophosphokinase, also called SulD Streptococcus pneumoniae