Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hyun, C.G.; Kim, S.S.; Suh, J.W.
    Identification of essential amino acid residues in valine dehydrogenase from Streptomyces albus (2006), J. Microbiol., 44, 50-53.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.8 C251S kcat for deamination is 13% of the wild-type value Streptomyces albus
1.4.1.8 C30S kcat for deamination is 13% of the wild-type value Streptomyces albus
1.4.1.8 V305L kcat for deamination is 0.1% of the wild-type value Streptomyces albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.8 0.091
-
 NAD+ pH 10.5, mutant enzyme C30S Streptomyces albus
1.4.1.8 0.15
-
 NAD+ pH 10.5, wild-type enzyme Streptomyces albus
1.4.1.8 0.159
-
 NAD+ pH 10.5, mutant enzyme C251S Streptomyces albus
1.4.1.8 0.37
-
 NAD+ pH 10.5, mutant enzyme V305L Streptomyces albus
1.4.1.8 0.8
-
 L-valine pH 10.5, mutant enzyme C30S Streptomyces albus
1.4.1.8 1.6
-
 L-valine pH 10.5, mutant enzyme C251S Streptomyces albus
1.4.1.8 1.686
-
 L-valine pH 10.5, wild-type enzyme Streptomyces albus
1.4.1.8 100
-
 L-valine pH 10.5, mutant enzyme V305L Streptomyces albus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.8 Streptomyces albus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.8
-
 Streptomyces albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.8 L-isoleucine + H2O + NAD+ 27.5% of the activity with L-valine Streptomyces albus 3-methyl-2-oxopentanoate + NADH + NH3
-
 ?
1.4.1.8 L-leucine + H2O + NAD+ 15% of the activity with L-valine Streptomyces albus 2-oxoisocaproate + NADH + NH3
-
 ?
1.4.1.8 L-norleucine + H2O + NAD+ 15% of the activity with L-valine Streptomyces albus 2-oxocaproate + NADH + NH3
-
 ?
1.4.1.8 L-norvaline + H2O + NAD+ as active as with L-valine Streptomyces albus 2-oxovalerate + NH3 + NADH
-
 ?
1.4.1.8 L-valine + NAD+ + H2O
-
 Streptomyces albus 2-oxoisovalerate + NH3 + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.8 ValDH
-
 Streptomyces albus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.8 NAD+
-
 Streptomyces albus
1.4.1.8 NADH
-
 Streptomyces albus