EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.2.2 | expression of GST-tagged SULT2B1 | Homo sapiens |
2.8.2.2 | expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b | Homo sapiens |
2.8.2.15 | expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.2.2 | cytosol | - |
Homo sapiens | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.2 | Mg2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7-oxocholesterol | Homo sapiens | SULT2B1b, SULT2B1a, and SULT2A1 | adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + cholesterol | Homo sapiens | SULT2B1b, SULT2B1a, and SULT2A1 | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Homo sapiens | SULT2A1, low activity with SULT2B1b and SULT2B1a | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.2 | Homo sapiens | - |
- |
- |
2.8.2.2 | Homo sapiens | O00204 | - |
- |
2.8.2.15 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.2 | recombinant GST-tagged SULT2B1 by glutathione affinity chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.2.2 | atheroma cell | - |
Homo sapiens | - |
2.8.2.2 | MCF-7 cell | - |
Homo sapiens | - |
2.8.2.2 | additional information | no expression in 293T cells | Homo sapiens | - |
2.8.2.15 | MCF-7 cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 0.0019 | - |
recombinant SULT2B1b, substrate 7beta-hydroxycholesterol | Homo sapiens |
2.8.2.2 | 0.0048 | - |
recombinant SULT2B1b, substrate cholesterol 7alpha-hydroperoxide | Homo sapiens |
2.8.2.2 | 0.0071 | - |
recombinant SULT2B1b, substrate 7-oxocholesterol | Homo sapiens |
2.8.2.2 | 0.0075 | - |
recombinant SULT2B1b, substrate cholesterol | Homo sapiens |
2.8.2.2 | 0.021 | - |
recombinant SULT2B1b, substrate 7alpha-hydroxycholesterol | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.2 | 3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7-oxo-cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7-oxocholesterol | SULT2B1b, SULT2B1a, and SULT2A1 | Homo sapiens | adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol | SULT2B1b, the 7alpha derivative is highly preferred | Homo sapiens | adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol | SULT2B1b, the 7alpha derivative is highly preferred | Homo sapiens | adenosine 3',5'-bisphosphate + 7beta-hydroxycholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + cholesterol | SULT2B1b, SULT2B1a, and SULT2A1 | Homo sapiens | adenosine 3',5'-bisphosphate + cholesterol 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + cholesterol 7alpha-hydroperoxide | SULT2B1b | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
2.8.2.2 | 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | SULT2A1, low activity with SULT2B1b and SULT2B1a | Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
2.8.2.2 | additional information | SULT2B1b sulfates oxosterols, overview | Homo sapiens | ? | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + 7-oxo-cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl sulfate | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesteryl sulfate | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 7beta-hydroxycholesteryl sulfate | - |
? | |
2.8.2.15 | 3'-phosphoadenylyl sulfate + cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + cholesteryl sulfate | - |
? | |
2.8.2.15 | additional information | SULT2B1b sulfates oxosterols, overview | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.2.2 | cholesterol sulfotransferase | - |
Homo sapiens |
2.8.2.2 | More | cf. EC 2.8.2.15 | Homo sapiens |
2.8.2.2 | steroid/sterol sulfotransferase | - |
Homo sapiens |
2.8.2.2 | SULT2A1 | - |
Homo sapiens |
2.8.2.2 | SULT2B1a | - |
Homo sapiens |
2.8.2.2 | SULT2B1b | - |
Homo sapiens |
2.8.2.15 | cholesterol sulfotransferase | - |
Homo sapiens |
2.8.2.15 | More | cf. EC 2.8.2.2 | Homo sapiens |
2.8.2.15 | steroid/sterol sulfotransferase | - |
Homo sapiens |
2.8.2.15 | SULT2B1b | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 7.1 | 7.5 | assay at | Homo sapiens |