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Literature summary extracted from

  • Carter, J.N.; Beatty, K.E.; Simpson, M.T.; Butler, A.
    Reactivity of recombinant and mutant vanadium bromoperoxidase from the red alga Corallina officinalis (2002), J. Inorg. Biochem., 91, 59-69.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.18 expression in Escherichia coli Corallina officinalis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.18 H480A results in the loss of the ability to efficiently oxidize bromide, but retains the ability to oxidize iodide Corallina officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.18 0.0012
-
 Br- pH 6.5, recombinant enzyme Corallina officinalis
1.11.1.18 0.0013
-
 Br- pH 6.5, native enzyme Corallina officinalis
1.11.1.18 0.017
-
 H2O2 pH 6.5, recombinant enzyme Corallina officinalis
1.11.1.18 0.027
-
 H2O2 pH 6.5, native enzyme Corallina officinalis
1.11.1.18 0.2
-
 H2O2 pH 6.5, mutant enzyme H480A Corallina officinalis
1.11.1.18 1.8
-
 I- recombinant enzyme Corallina officinalis
1.11.1.18 33
-
 I- mutant enzyme H480A Corallina officinalis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.18 Corallina officinalis Q8LLW7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.18
-
 Corallina officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.18 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Corallina officinalis ?
-
 ?
1.11.1.18 I- + H2O2
-
 Corallina officinalis triiodide + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 CVBPO
-
 Corallina officinalis
1.11.1.18 vanadium bromoperoxidase
-
 Corallina officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.18 6 6.5 maximum bromoperoxidase activity of mutant H480A under conditions of high substrate concentrations (5 mM H2O2 and 100 mM Br-), however with reduced specific activity as compared to recombinant wild-type enzyme Corallina officinalis
1.11.1.18 6
-
 optimal bromoperoxidase activity of recombinant wild-type enzyme using low substrate concentrations (0.5 mM H2O2, 5 mM Br-) and high substrate concentrations (5 mM H2O2, 100 mM Br-) Corallina officinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.18 5 7 pH 5.0: about 35% of maximal activity, pH 7.0: about 25% of maximal activity Corallina officinalis