Any feedback?
Literature summary extracted from
- Purification, characterization, and crystallization of the components of a biphenyl dioxygenase system from Sphingobium yanoikuyae B1 (2007), J. Ind. Microbiol. Biotechnol., 34, 311-324.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.12.18 | expressed in Escherichia coli strain BL21Star | Sphingobium yanoikuyae |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.12.18 | hanging drop vapour diffusion method using 20% (w/v) polyethylene glycol 3350, 1 M NaCl and 0.1 M ZnCl2 at 6°C | Sphingobium yanoikuyae |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.18 | Iron | exogenous ferrous iron is required for maximal activity, the oxygenase component contains 2.43 g/mol iron, the ferredoxin component contains 2.1 g/mol iron | Sphingobium yanoikuyae |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 12000 | - |
ferredoxin component, SDS-PAGE | Sphingobium yanoikuyae |
| 1.14.12.18 | 43000 | - |
reductase component, SDS-PAGE | Sphingobium yanoikuyae |
| 1.14.12.18 | 217000 | - |
oxygenase component, SDS-PAGE | Sphingobium yanoikuyae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.18 | Sphingobium yanoikuyae | - |
formerly Beijerinckia sp. and Sphingomonas yanoikuyae | - |
| 1.14.12.18 | Sphingobium yanoikuyae B1 | - |
formerly Beijerinckia sp. and Sphingomonas yanoikuyae | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.18 | butyl-Sepharose XK26/40 column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography | Sphingobium yanoikuyae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 28.9 | - |
oxygenase component, crude cell extract | Sphingobium yanoikuyae |
| 1.14.12.18 | 217 | - |
oxygenase component, after 10fold purification | Sphingobium yanoikuyae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.18 | benzo[a]pyrene + NADH + O2 | - |
Sphingobium yanoikuyae | ? | - |
? | |
| 1.14.12.18 | benzo[a]pyrene + NADH + O2 | - |
Sphingobium yanoikuyae B1 | ? | - |
? | |
| 1.14.12.18 | biphenyl + NAD(P)H + O2 | - |
Sphingobium yanoikuyae | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| 1.14.12.18 | biphenyl + NAD(P)H + O2 | - |
Sphingobium yanoikuyae B1 | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| 1.14.12.18 | naphthalene + NADH + O2 | - |
Sphingobium yanoikuyae | ? | - |
? | |
| 1.14.12.18 | naphthalene + NADH + O2 | - |
Sphingobium yanoikuyae B1 | ? | - |
? | |
| 1.14.12.18 | phenanthrene + NADH + O2 | - |
Sphingobium yanoikuyae | ? | - |
? | |
| 1.14.12.18 | phenanthrene + NADH + O2 | - |
Sphingobium yanoikuyae B1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.12.18 | heterotrimer | oxygenase component, MALDI-TOF | Sphingobium yanoikuyae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.12.18 | biphenyl dioxygenase | - |
Sphingobium yanoikuyae |
| 1.14.12.18 | BPDO | - |
Sphingobium yanoikuyae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.18 | NADH | - |
Sphingobium yanoikuyae | |
| 1.14.12.18 | NADPH | - |
Sphingobium yanoikuyae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
