Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khatri, A.; Zhang, B.; Doherty, E.; Chapman, J.; Ow, K.; Pwint, H.; Martiniello-Wilks, R.; Russell, P.J.
    Combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice (2006), J. Gene Med., 8, 1086-1096.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expression of the fusion gene CDUPRT, combining cytosine deaminase combined with uracil phosphoribosyl transferase, in RM1 cancer cells in a gene therapeutic approach, overview Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.9 additional information expression of fusion protein cytosine deaminase combined with uracil phosphoribosyl transferase leads to local and distant bystander effects against intraprostatic mouse androgen-refractory prostate, RM1, tumors in immunocompetent mice, CDUPRT-GDEPT expression significantly suppresses the aggressive growth of RM1 prostate tumors and lung pseudo-metastases via immune mechanisms involving necrosis and apoptosis, overview Mus musculus
3.5.4.1 additional information construction of a fusion gene using cytosine deaminase in combination with uracil phosphoribosyl transferase, the product of the suicide gene, CDUPRT, converts the prodrug, 5-fluorocytosine, into 5-fluorouracil and other cytotoxic metabolites that kill both CDUPRT-expressing and surrounding cells, via a bystander effect, in pseudo-metastates in mouse tissues, tissue-specifix effects, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate Mus musculus recombinant fusion enzyme in RM1 tumors of lung, spleen, heart, and prostate 5-fluoro-UMP + diphosphate
-
 ?
3.5.4.1 5-fluorocytosine + H2O Escherichia coli recombinant enzyme, combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice in gene-directed enzyme prodrug therapy, overview 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Mus musculus
-
-
-
3.5.4.1 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate recombinant fusion enzyme in RM1 tumors of lung, spleen, heart, and prostate Mus musculus 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate recombinant fusion enzyme Mus musculus 5-fluoro-UMP + diphosphate
-
 ?
3.5.4.1 5-fluorocytosine + H2O recombinant enzyme, combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice in gene-directed enzyme prodrug therapy, overview Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 5-fluorocytosine + H2O activation of the prodrug Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 uracil phosphoribosyl transferase
-
 Mus musculus