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Literature summary extracted from

  • Alkhalfioui, F.; Renard, M.; Montrichard, F.
    Unique properties of NADP-thioredoxin reductase C in legumes (2006), J. Exp. Bot., 58, 969-978.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9
-
 Medicago truncatula
1.8.1.9 expressed in Escherichia coli BL21 (pLysS) cells Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0024
-
 NADPH
-
 Medicago truncatula
1.8.1.9 0.0024
-
 NADPH pH 7.5 Medicago truncatula
1.8.1.9 0.0095
-
 BAS1
-
 Medicago truncatula
1.8.1.9 0.011
-
 NADH
-
 Medicago truncatula
1.8.1.9 0.011
-
 NADH pH 7.5 Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 chloroplast
-
 Medicago truncatula 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 52000
-
 reducing SDS-PAGE, with His-tag Medicago truncatula
1.8.1.9 52000
-
 3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE Medicago truncatula
1.8.1.9 148000
-
 gel filtration Medicago truncatula
1.8.1.9 148000
-
 Superdex 200 gel filtration Medicago truncatula
1.8.1.9 150000
-
 non-reducing SDS-PAGE Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Medicago truncatula
-
-
-
1.8.1.9 Medicago truncatula A6XJ27
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni2+-chelating Sepharose column chromatography and Superdex 75 column gel filtration Medicago truncatula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 cotyledon from dry and germinating seeds Medicago truncatula
-
1.8.1.9 leaf
-
 Medicago truncatula
-
1.8.1.9 leaf NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination Medicago truncatula
-
1.8.1.9 seed more abundant in cotyledons from dry and germinating seeds Medicago truncatula
-
1.8.1.9 stem
-
 Medicago truncatula
-
1.8.1.9 stem NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Medicago truncatula 2-nitro-5-thiobenzoate + NAD+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Medicago truncatula 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Medicago truncatula 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 BAS1 + hydrogen peroxide
-
 Medicago truncatula ?
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Medicago truncatula thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin disulfide + NADH + H+
-
 Medicago truncatula thioredoxin + NAD+
-
 r
1.8.1.9 thioredoxin disulfide + NADH + H+ MtNTRC can use either NADPH or NADH as cofactors Medicago truncatula thioredoxin + NAD+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+ MtNTRC can use either NADPH or NADH as cofactors Medicago truncatula thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 polymer in non-reducing SDS-PAGE, NTRC appears as a single band of 150000 Da corresponding to a polymer probably comprising three subunits linked by disulphide bridges Medicago truncatula
1.8.1.9 trimer 3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 MtNTRC
-
 Medicago truncatula
1.8.1.9 NADP-thioredoxin reductase C
-
 Medicago truncatula
1.8.1.9 NTR
-
 Medicago truncatula
1.8.1.9 NTRC
-
 Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.62
-
 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Medicago truncatula
1.8.1.9 0.62
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.5 Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADH
-
 Medicago truncatula
1.8.1.9 NADPH
-
 Medicago truncatula