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Literature summary extracted from

  • Jeon, W.B.; Singer, S.W.; Ludden, P.W.; Rubio, L.M.
    New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster (2005), J. Biol. Inorg. Chem., 10, 903-912.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.7.4 C338A complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ Rhodospirillum rubrum
1.2.7.4 C451A complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ Rhodospirillum rubrum
1.2.7.4 C451S 1.4% of wild-type specific activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ Rhodospirillum rubrum
1.2.7.4 C481A unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ Rhodospirillum rubrum
1.2.7.4 C531A 0.1% of wild-type specific activity Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.4 0.024
-
 CO mutant C451S, pH 7.5 Rhodospirillum rubrum
1.2.7.4 0.032
-
 CO wild-type, pH 7.5 Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.4 membrane
-
 Rhodospirillum rubrum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Iron wild-type, 8.97 mol per mol of enzyme Rhodospirillum rubrum
1.2.7.4 Nickel wild-type, 0.85 mol per mol of enzyme. Analysis of activation of wild-type and mutant apo-enzymes by nickel Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Rhodospirillum rubrum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 6.9
-
 mutant C531A, pH 7.5 Rhodospirillum rubrum
1.2.7.4 86.3
-
 mutant C451S, pH 7.5 Rhodospirillum rubrum
1.2.7.4 6230
-
 wild-type, pH 7.5 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + methyl viologen
-
 Rhodospirillum rubrum CO2 + reduced methyl viologen
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 8.4
-
 mutant C451S Rhodospirillum rubrum
1.2.7.4 8.6
-
 wild-type Rhodospirillum rubrum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.7.4 6.5 9.5 wild-type and mutants apo-enzymes, stable for at least 30 min within this range Rhodospirillum rubrum