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Literature summary extracted from

  • Ryder, J.W.; Lau, K.S.; Kamm, K.E.; Stull, J.T.
    Enhanced skeletal muscle contraction with myosin light chain phosphorylation by a calmodulin-sensing kinase (2007), J. Biol. Chem., 282, 20447-20454.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 Calmodulin a calmodulin-sensitive MLCK isozyme Oryctolagus cuniculus
2.7.11.18 additional information electric stimulation of intact skeletal muscles activate the enzyme activity, overview Oryctolagus cuniculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression in transgenic mice, expression in COS cells Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 additional information construction of transgenic mice that express an skeletal muscle-MLCK, i.e. skMLCK, CaM biosensor in skeletal muscle to determine whether skMLCK or calmodulin is limiting to twitch force potentiation. Three transgenic mouse lines exhibited up to 22-fold increases in skMLCK protein expression in fast-twitch extensor digitorum longus muscle containing type IIa and IIb fibers, with comparable expressions in slow-twitch soleus muscle containing type I and IIa fibers, overview Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ dependent on Oryctolagus cuniculus
2.7.11.18 Mg2+
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + light chain myosin II Oryctolagus cuniculus MLC phosphorylation increases muscle contraction and plays a prominent role in skeletal muscle force potentiation of fast-twitch type IIb but not type I or IIa fibers, overview ADP + phosphorylated light chain myosin II
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + light chain myosin II MLC phosphorylation increases muscle contraction and plays a prominent role in skeletal muscle force potentiation of fast-twitch type IIb but not type I or IIa fibers, overview Oryctolagus cuniculus ADP + phosphorylated light chain myosin II
-
 ?
2.7.11.18 ATP + light chain myosin II regulatory myosin chain Oryctolagus cuniculus ADP + phosphorylated light chain myosin II
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Oryctolagus cuniculus
2.7.11.18 More cf. EC 2.7.11.17 Oryctolagus cuniculus
2.7.11.18 myosin light chain kinase
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Oryctolagus cuniculus