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Literature summary extracted from

  • Nagle, C.A.; An, J.; Shiota, M.; Torres, T.P.; Cline, G.W.; Liu, Z.X.; Wang, S.; Catlin, R.L.; Shulman, G.I.; Newgard, C.B.; Coleman, R.A.
    Hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance (2007), J. Biol. Chem., 282, 14807-14815.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.15 medicine major finding of the study is that overexpressing GPAT in liver causes hepatic steatosis and hepatic insulin resistance in the absence of obesity or high-fat feeding Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 hepatic overexpression of the enzyme with an adenoviral construct Rattus norvegicus
2.3.1.15 recombinant GPAT1-FLAG adenovirus and Ad-EGFP are constructed Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Rattus norvegicus
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Rattus norvegicus
-
2.3.1.15 liver hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance Rattus norvegicus
-
2.3.1.15 muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.0003
-
 activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-EGFP Rattus norvegicus
2.3.1.15 0.00083
-
 activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-GPAT1 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 fatty acyl-CoA + glycerol-3-phosphate
-
 Rattus norvegicus CoA + lysophosphatidic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 acyl-CoA:glycerol-3-phosphate acyltransferase 1
-
 Rattus norvegicus
2.3.1.15 Ad-GPAT1 adenoviral GPAT1 construct Rattus norvegicus
2.3.1.15 glycerol-sn-3-phosphate acyltransferase 1
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 20
-
 activity assay at room temperature Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.5
-
 activity assay Rattus norvegicus