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Literature summary extracted from

  • Zhao, T.J.; Yan, Y.B.; Liu, Y.; Zhou, H.M.
    The generation of the oxidized form of creatine kinase is a negative regulation on muscle creatine kinase (2007), J. Biol. Chem., 282, 12022-12029.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2
-
 Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 C146S enzyme preparation contains only reduced form Oryctolagus cuniculus
2.7.3.2 C254S enzyme preparation contains both oxidized and reduced forms Oryctolagus cuniculus
2.7.3.2 C283S enzyme preparation contains both oxidized and reduced forms Oryctolagus cuniculus
2.7.3.2 C74S enzyme preparation contains only reduced form Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.37
-
 ATP reduced form of creatine kinase Oryctolagus cuniculus
2.7.3.2 0.38
-
 ATP wild-type containing both oxidized and reduced form Oryctolagus cuniculus
2.7.3.2 0.43
-
 ATP oxidized form of creatine kinase Oryctolagus cuniculus
2.7.3.2 8.33
-
 Creatine reduced form of creatine kinase Oryctolagus cuniculus
2.7.3.2 8.39
-
 Creatine wild-type containing both oxidized and reduced form Oryctolagus cuniculus
2.7.3.2 8.48
-
 Creatine oxidized form of creatine kinase Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Oryctolagus cuniculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.3.2 side-chain modification intrachain disulfide bond in oxidized form of enzyme is formed between C74 and C146 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle oxidized form of enzyme Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 210
-
 oxidized form of creatine kinase Oryctolagus cuniculus
2.7.3.2 261
-
 wild-type containing both oxidized and reduced form Oryctolagus cuniculus
2.7.3.2 274.9
-
 reduced form of creatine kinase Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Oryctolagus cuniculus ADP + creatine phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.2 More tertiary structure of oxidized form of enzyme is more easily disturbed than reduced form. The oxidized form, unlike reduced form, cannot interact with the M-line protein myomesin and therefore might have no role in muscle contraction. Oxidized creatine kinase can be rapidly ubiquitinated by muscle ring finger protein I and may be substrate of the ATP-ubiquitin-proteasome pathway Oryctolagus cuniculus