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Literature summary extracted from

  • Lack, G.; Homberger-Zizzari, E.; Folkers, G.; Scapozza, L.; Perozzo, R.
    Recombinant expression and biochemical characterization of the unique elongating beta-ketoacyl-acyl carrier protein synthase involved in fatty acid biosynthesis of Plasmodium falciparum using natural and artificial substrates (2006), J. Biol. Chem., 281, 9538-9546.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.41 drug development the enzyme is a target for antibacterial drugs Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 gene fabBF, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble NusA-fusion protein FabBF in Escherichia coli strain BL21(DE3) Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin irreversible inhibition, IC50: 0.0158 mM Plasmodium falciparum
2.3.1.41 thiolactomycin competitive inhibition, IC50: 0.0236 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 additional information
-
 additional information kinetics Plasmodium falciparum
2.3.1.41 0.116
-
 C6-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.196
-
 C12-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.198
-
 C8-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.211
-
 C4-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.403
-
 C10-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.437
-
 C14-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Plasmodium falciparum
-
 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Plasmodium falciparum
-
 FabF/FabH; gene fabBF, encoding the only elongating beta-ketoacyl-ACP synthase in the organism
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant soluble NusA-fusion protein FabBF from Escherichia coli strain BL21(DE3) by and gel filtration Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 additional information
-
-
 Plasmodium falciparum
2.3.1.41 0.105
-
 purified recombinant enzyme with substrate C6-[acyl-carrier-protein] Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] C4 to C14 acyl-[ACP]s, the enzyme readily elongates C4:0-through C10:0-PfACPs, has significantly less activity with C12:0- and C14:0-ACPs, and no capacity for elongation of C16:0-ACP and C18:0-ACP Plasmodium falciparum 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C10-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum C12-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C12-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum C14-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C14-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum C16-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C4-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum C6-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C6-[acyl-carrier protein] + malonyl-[acyl-carrier protein] best substrate Plasmodium falciparum C8-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 C8-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Plasmodium falciparum C10-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 elongating beta-ketoacyl-acyl carrier protein synthase
-
 Plasmodium falciparum
2.3.1.41 FabBF
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 37
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.41 0.69
-
 dodecanoyl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.69
-
 C12-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.96
-
 hexanoyl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 0.96
-
 C6-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.28
-
 tetradecanoyl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.28
-
 C14-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.32
-
 butyryl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.32
-
 C4-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.63
-
 octanoyl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 1.63
-
 C8-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 3.07
-
 decanoyl-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
2.3.1.41 3.07
-
 C10-[acyl-carrier protein] pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 7.5
-
 assay at Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.41 0.01
-
 thiolactomycin pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.41 0.0158
-
 irreversible inhibition, IC50: 0.0158 mM Plasmodium falciparum cerulenin
2.3.1.41 0.0236
-
 competitive inhibition, IC50: 0.0236 mM Plasmodium falciparum thiolactomycin