Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zufferey, R.; Ben Mamoun, C.
    Leishmania major expresses a single dihydroxyacetone phosphate acyltransferase localized in the glycosome, important for rapid growth and survival at high cell density and essential for virulence (2006), J. Biol. Chem., 281, 7952-7959.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.42 gene DAT, DNA amd amino acid sequence determination and analysis, DAT functionally complements the lethality resulting from the loss of both dihydroxyacetone phosphate and glycerol-3-phosphate acyltransferase activities in yeast, recombinant DAT exhibits biochemical properties similar to those of the native enzyme of the promastigote stage parasites Leishmania major

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.42 additional information construction of a DELTAmdat/DELTAlmdat null mutant results in complete abrogation of the parasite dihydroxyacetone phosphate acyltransferase activity, lack of LmDAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice, phenotype, overview Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.42 additional information
-
 additional information Michaelis-Menten kinetics Leishmania major
2.3.1.42 0.69
-
 dihydroxyacetone phosphate pH 7.5, 30°C, recombinant enzyme Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 glycosome
-
 Leishmania major 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.42 Mg2+
-
 Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.42 dihydroxyacetone phosphate + acyl-CoA Leishmania major the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice 1-acyl-glycerone phosphate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Leishmania major
-
 gene DAT
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 additional information promastigote stage parasites Leishmania major
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 dihydroxyacetone phosphate + acyl-CoA the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice Leishmania major 1-acyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + arachidoyl-CoA
-
 Leishmania major 1-arachidoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + lauroyl-CoA
-
 Leishmania major 1-lauroyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + linoleoyl-CoA
-
 Leishmania major 1-linoleoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + myristoyl-CoA
-
 Leishmania major 1-myristoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + oleoyl-CoA
-
 Leishmania major 1-oleoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + palmitoleoyl-CoA
-
 Leishmania major 1-palmitoleoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + palmitoyl-CoA palmitoyl-CoA is the preferred acyl-CoA substrate Leishmania major palmitoyl-glycerone phosphate + CoA
-
 ?
2.3.1.42 dihydroxyacetone phosphate + stearoyl-CoA
-
 Leishmania major 1-stearoyl-glycerone phosphate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.42 DHAP acyltransferase
-
 Leishmania major
2.3.1.42 DHAPAT
-
 Leishmania major
2.3.1.42 dihydroxyacetone phosphate acyltransferase
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 30
-
 assay at Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.42 7.5
-
 assay at Leishmania major