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Literature summary extracted from

  • Zhou, W.; Lepesheva, G.I.; Waterman, M.R.; Nes, W.D.
    Mechanistic analysis of a multiple product sterol methyltransferase implicated in ergosterol biosynthesis in Trypanosoma brucei (2006), J. Biol. Chem., 281, 6290-6296.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.41 drug development inhibition studies as a powerful approach to rational design of new anti-sleeping sickness chemotherapeutic drugs Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.41 overexpressed in Escherichia coli HMS-174 cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.41 24(R,S),25-epiminolanosterol
-
 Trypanosoma brucei
2.1.1.41 25-azalanosterol
-
 Trypanosoma brucei
2.1.1.41 26,27-dehydrozymosterol
-
 Trypanosoma brucei
2.1.1.41 ergosterol
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.047
-
 Zymosterol
-
 Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.41 40000
-
 4 * 40000, SDS-PAGE Trypanosoma brucei
2.1.1.41 160000
-
 gel filtration Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.41 to homogeneity Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 additional information neither lanosterol nor other C4-methyl-containing sterols bind productively to the enzyme Trypanosoma brucei ?
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine C4-demethyl sterols with double bonds in the nucleus at positions 8, 7, or 5 are acceptable substrates Trypanosoma brucei fecosterol + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.41 tetramer 4 * 40000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.1.1.41 SMT
-
 Trypanosoma brucei
2.1.1.41 sterol methyltransferase
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.41 35 40
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.41 0.013
-
 Zymosterol
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.41 7.5
-
-
 Trypanosoma brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.41 6.8 7.5
-
 Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.41 0.000039
-
 25-azalanosterol
-
 Trypanosoma brucei
2.1.1.41 0.000049
-
 24(R,S),25-epiminolanosterol
-
 Trypanosoma brucei
2.1.1.41 0.027
-
 ergosterol
-
 Trypanosoma brucei
2.1.1.41 0.029
-
 26,27-dehydrozymosterol
-
 Trypanosoma brucei