EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.11 | medicine | target for antibiotic development | Streptococcus pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.11 | expression as His-tag fusion protein in Escherichia coli BL21 (DE3) | Streptococcus pneumoniae |
1.2.1.11 | into a pET28a vector for expression in Escherichia coli BL21DE3 | Streptococcus pneumoniae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.11 | - |
Streptococcus pneumoniae |
1.2.1.11 | crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde | Streptococcus pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.11 | 3.3 | - |
NADPH | - |
Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.11 | L-aspartate 4-semialdehyde + phosphate + NADP+ | Streptococcus pneumoniae | - |
L-4-aspartyl phosphate + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.11 | Streptococcus pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.11 | Ni-affinity chromatography and anion exchange chromatography | Streptococcus pneumoniae |
1.2.1.11 | using a Co2+ affinity matrix and anion exchange chromatography | Streptococcus pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.11 | L-4-aspartyl phosphate + NADPH | - |
Streptococcus pneumoniae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
1.2.1.11 | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Streptococcus pneumoniae | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.11 | homodimer | crystallization | Streptococcus pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.11 | ASADH | - |
Streptococcus pneumoniae |
1.2.1.11 | aspartate-beta-semialdehyde dehydrogenase | - |
Streptococcus pneumoniae |
1.2.1.11 | aspartate-semialdehyde dehydrogenase | - |
Streptococcus pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.11 | 22 | - |
activity assay performed at room temperature | Streptococcus pneumoniae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.11 | 2 | - |
L-aspartate-semialdehyde | - |
Streptococcus pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.11 | 9 | - |
activity assay | Streptococcus pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.11 | NAD+ | in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ | Streptococcus pneumoniae | |
1.2.1.11 | NADP+ | - |
Streptococcus pneumoniae | |
1.2.1.11 | NADP+ | in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ | Streptococcus pneumoniae | |
1.2.1.11 | NADPH | - |
Streptococcus pneumoniae |