EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.84 | gene hapE, expression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.84 | H61T | the H61T mutant is purified as apo-enzyme and mainly exists as a dimeric species, the binding of FAD to the enzyme restores the octameric conformation | Pseudomonas fluorescens |
1.14.13.84 | R339A | site-directed mutagenesiss, the mutant shows decreased activity and affinity to NADPH compared to the wild-type enzyme, the mutant only weakly interacts with 3-aminopyridine adenine dinucleotide phosphate | Pseudomonas fluorescens |
1.14.13.84 | R440A | site-directed mutagenesiss, inactive mutant, the mutant shows highly increased affinity to NADPH compared to the wild-type enzyme | Pseudomonas fluorescens |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.84 | the association with the coenzyme NADPH is crucial for enzyme stability, 3-aminopyridine adenine dinucleotide phosphate highly stabilizes the inactive dimeric state of the enzyme | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.84 | 3-Aminopyridine adenine dinucleotide phosphate | cofactor analogue, tight binding to the wild-type enzyme and mutant R440A | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.84 | 145100 | - |
mutant R339A, dimeric structure, mass spectrometry | Pseudomonas fluorescens |
1.14.13.84 | 145100 | - |
mutant R440A, dimeric structure, mass spectrometry | Pseudomonas fluorescens |
1.14.13.84 | 145200 | - |
wild-type enzyme, dimeric structure, mass spectrometry | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.84 | (4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | (4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens ACB | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.84 | Pseudomonas fluorescens | - |
gene hapE | - |
1.14.13.84 | Pseudomonas fluorescens ACB | - |
gene hapE | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.84 | (4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O | reaction mechanism | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.84 | (4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | (4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | additional information | the enzyme catalyzes Baeyer-Villiger oxidations of a wide range of ketones, thereby generating esters or lactones, overview | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.84 | additional information | the enzyme catalyzes Baeyer-Villiger oxidations of a wide range of ketones, thereby generating esters or lactones, overview | Pseudomonas fluorescens ACB | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.84 | dimer | - |
Pseudomonas fluorescens |
1.14.13.84 | More | quaternary structure of wild-type and mutant enzymes, overview | Pseudomonas fluorescens |
1.14.13.84 | octamer | - |
Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.84 | HAPMO | - |
Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.84 | FAD | required for activity, each subunit contains a non-covalently, tightly bound FAD cofactor, binding of FAD is important for the octameric conformation | Pseudomonas fluorescens | |
1.14.13.84 | additional information | complex formation between the cofactors NADPH or 3-aminopyridine adenine dinucleotide phosphate | Pseudomonas fluorescens | |
1.14.13.84 | NADPH | - |
Pseudomonas fluorescens |