EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.99.6 | mutant enzymes expressed in Escherichia coli | Cupriavidus necator |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.12.99.6 | F110L | 18% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
1.12.99.6 | I62V | 6% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
1.12.99.6 | I62V/F110L | mutant enzyme shows no H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.99.6 | Cupriavidus necator | - |
- |
- |
1.12.99.6 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.99.6 | oxygen tolerance is based on limited access of oxygen to the active site | Cupriavidus necator |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.99.6 | - |
Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.99.6 | H2 + acceptor | - |
Cupriavidus necator | H+ + reduced acceptor | - |
? | |
1.12.99.6 | H2 + acceptor | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.99.6 | H2-sensing [NiFe] hydrogenase | - |
Cupriavidus necator |