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Literature summary extracted from
- Studies on the 4-carbon precursor in the biosynthesis of riboflavin. Purification and properties of L-3,4-dihydroxy-2-butanone-4-phosphate synthase (1990), J. Biol. Chem., 265, 19479-19485.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.12 | EDTA | complete inhibition, reversible by Mg2+ | Meyerozyma guilliermondii |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.12 | Mg2+ | required for activity | Meyerozyma guilliermondii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 24000 | - |
1 * 24000, SDS-PAGE | Meyerozyma guilliermondii |
| 4.1.99.12 | 30000 | - |
gel filtration | Meyerozyma guilliermondii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | Meyerozyma guilliermondii | a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.12 | Meyerozyma guilliermondii | - |
i.e. Pichia guilliermondii | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | native enzyme to homogeneity, by anion and cation exchange chromatography, hydroxyapatite chromatography, and gel filtration | Meyerozyma guilliermondii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 14 | - |
purified enzyme | Meyerozyma guilliermondii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | - |
Meyerozyma guilliermondii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | product identification by NMR and CD spectroscopy | ? | |
| 4.1.99.12 | D-ribulose 5-phosphate | a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X | Meyerozyma guilliermondii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | monomer | 1 * 24000, SDS-PAGE | Meyerozyma guilliermondii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | L-3,4-dihydroxy-2-butanone-4-phosphate synthase | - |
Meyerozyma guilliermondii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 37 | - |
assay at | Meyerozyma guilliermondii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 7.5 | - |
assay at | Meyerozyma guilliermondii |
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