BRENDA - Enzyme Database

Sphingosine kinase: biochemical and cellular regulation and role in disease

Taha, T.A.; Hannun, Y.A.; Obeid, L.M.; J. Biochem. Mol. Biol. 39, 113-131 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.91
cardiolipin
;
Homo sapiens
2.7.1.91
additional information
not affected by bovine serum albumin; not affected by bovine serum albumin; not stimulated by phosphatidylcholine or phosphatidylethanolamine
Homo sapiens
2.7.1.91
phosphatidic acid
;
Homo sapiens
2.7.1.91
phosphatidylinositol
;
Homo sapiens
2.7.1.91
phosphatidylinositol bisphosphate
;
Homo sapiens
2.7.1.91
phosphatidylserine
;
Homo sapiens
2.7.1.91
Triton X-100
;
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.91
bovine serum albumin
-
Homo sapiens
2.7.1.91
Cu2+
-
Homo sapiens
2.7.1.91
dimethylsphingosine
noncompetitive inhibitor
Homo sapiens
2.7.1.91
DL-threo-dihydrosphinganine
competitive inhibitor
Homo sapiens
2.7.1.91
DL-threo-dihydrosphingosine
competitive inhibitor
Homo sapiens
2.7.1.91
Fe2+
-
Homo sapiens
2.7.1.91
KCl
increasing KCl concentration profoundly inhibits activity
Homo sapiens
2.7.1.91
NaCl
increasing NaCl concentration profoundly inhibits activity
Homo sapiens
2.7.1.91
Triton X-100
-
Homo sapiens
2.7.1.91
Zn2+
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.91
0.005
-
sphingosine
-
Rattus norvegicus
2.7.1.91
0.0138
-
sphingosine
-
Homo sapiens
2.7.1.91
0.0156
-
sphingosine
-
Homo sapiens
2.7.1.91
0.07
0.1
ATP
-
Homo sapiens
2.7.1.91
0.093
-
ATP
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.91
cytosol
;
Homo sapiens
5829
-
2.7.1.91
cytosol
-
Rattus norvegicus
5829
-
2.7.1.91
nucleus
;
Homo sapiens
5634
-
2.7.1.91
nucleus
-
Rattus norvegicus
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.91
K+
increasing KCl concentration stimulates activity
Homo sapiens
2.7.1.91
Mg2+
highest activity in the presence of Mg2+
Homo sapiens
2.7.1.91
Mn2+
-
Homo sapiens
2.7.1.91
Na+
increasing NaCl concentration stimulates activity
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.91
Homo sapiens
Q9NRA0
-
-
2.7.1.91
Homo sapiens
Q9NYA1
-
-
2.7.1.91
Rattus norvegicus
Q91V26
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.91
brain
-
Rattus norvegicus
-
2.7.1.91
heart
-
Rattus norvegicus
-
2.7.1.91
kidney
-
Rattus norvegicus
-
2.7.1.91
liver
-
Rattus norvegicus
-
2.7.1.91
lung
-
Rattus norvegicus
-
2.7.1.91
platelet
;
Homo sapiens
-
2.7.1.91
spleen
-
Rattus norvegicus
-
2.7.1.91
thymus
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.91
ATP + DL-threo-dihydrosphingosine
-
674380
Homo sapiens
ADP + DL-threo-dihydrosphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + FTY720
-
674380
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.91
ATP + phytosphingosine
-
674380
Homo sapiens
ADP + phytosphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphinganine
-
674380
Homo sapiens
ADP + sphinganine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphinganine
SK2 has a higher substrate affinity to sphinganine than to sphingosine
674380
Homo sapiens
ADP + sphinganine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphingosine
-
674380
Homo sapiens
ADP + sphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphingosine
-
674380
Rattus norvegicus
ADP + sphingosine 1-phosphate
-
-
-
?
2.7.1.91
additional information
shows no activity towards DL-threo-dihydrosphingosine, dimethylsphingosine, C2-ceramide, dioleoylacylglycerol, or phosphatidylserine
674380
Homo sapiens
?
-
-
-
-
2.7.1.91
additional information
shows no activity towards DL-threo-dihydrosphingosine, dimethylsphingosine, C2-ceramide, dioleoylacylglycerol, or phosphatidylserine
674380
Rattus norvegicus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.91
7.4
-
;
Homo sapiens
2.7.1.91
7.4
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.91
ATP
-
Rattus norvegicus
2.7.1.91
ATP
;
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.91
cardiolipin
-
Homo sapiens
2.7.1.91
additional information
not affected by bovine serum albumin
Homo sapiens
2.7.1.91
additional information
not affected by bovine serum albumin; not stimulated by phosphatidylcholine or phosphatidylethanolamine
Homo sapiens
2.7.1.91
phosphatidic acid
-
Homo sapiens
2.7.1.91
phosphatidylinositol
-
Homo sapiens
2.7.1.91
phosphatidylinositol bisphosphate
-
Homo sapiens
2.7.1.91
phosphatidylserine
-
Homo sapiens
2.7.1.91
Triton X-100
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.91
ATP
-
Rattus norvegicus
2.7.1.91
ATP
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.91
bovine serum albumin
-
Homo sapiens
2.7.1.91
Cu2+
-
Homo sapiens
2.7.1.91
dimethylsphingosine
noncompetitive inhibitor
Homo sapiens
2.7.1.91
DL-threo-dihydrosphinganine
competitive inhibitor
Homo sapiens
2.7.1.91
DL-threo-dihydrosphingosine
competitive inhibitor
Homo sapiens
2.7.1.91
Fe2+
-
Homo sapiens
2.7.1.91
KCl
increasing KCl concentration profoundly inhibits activity
Homo sapiens
2.7.1.91
NaCl
increasing NaCl concentration profoundly inhibits activity
Homo sapiens
2.7.1.91
Triton X-100
-
Homo sapiens
2.7.1.91
Zn2+
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.91
0.005
-
sphingosine
-
Rattus norvegicus
2.7.1.91
0.0138
-
sphingosine
-
Homo sapiens
2.7.1.91
0.0156
-
sphingosine
-
Homo sapiens
2.7.1.91
0.07
0.1
ATP
-
Homo sapiens
2.7.1.91
0.093
-
ATP
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.91
cytosol
-
Homo sapiens
5829
-
2.7.1.91
cytosol
-
Rattus norvegicus
5829
-
2.7.1.91
nucleus
-
Homo sapiens
5634
-
2.7.1.91
nucleus
-
Rattus norvegicus
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.91
K+
increasing KCl concentration stimulates activity
Homo sapiens
2.7.1.91
Mg2+
highest activity in the presence of Mg2+
Homo sapiens
2.7.1.91
Mn2+
-
Homo sapiens
2.7.1.91
Na+
increasing NaCl concentration stimulates activity
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.91
brain
-
Rattus norvegicus
-
2.7.1.91
heart
-
Rattus norvegicus
-
2.7.1.91
kidney
-
Rattus norvegicus
-
2.7.1.91
liver
-
Rattus norvegicus
-
2.7.1.91
lung
-
Rattus norvegicus
-
2.7.1.91
platelet
-
Homo sapiens
-
2.7.1.91
spleen
-
Rattus norvegicus
-
2.7.1.91
thymus
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.91
ATP + DL-threo-dihydrosphingosine
-
674380
Homo sapiens
ADP + DL-threo-dihydrosphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + FTY720
-
674380
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.91
ATP + phytosphingosine
-
674380
Homo sapiens
ADP + phytosphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphinganine
-
674380
Homo sapiens
ADP + sphinganine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphinganine
SK2 has a higher substrate affinity to sphinganine than to sphingosine
674380
Homo sapiens
ADP + sphinganine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphingosine
-
674380
Homo sapiens
ADP + sphingosine 1-phosphate
-
-
-
?
2.7.1.91
ATP + sphingosine
-
674380
Rattus norvegicus
ADP + sphingosine 1-phosphate
-
-
-
?
2.7.1.91
additional information
shows no activity towards DL-threo-dihydrosphingosine, dimethylsphingosine, C2-ceramide, dioleoylacylglycerol, or phosphatidylserine
674380
Homo sapiens
?
-
-
-
-
2.7.1.91
additional information
shows no activity towards DL-threo-dihydrosphingosine, dimethylsphingosine, C2-ceramide, dioleoylacylglycerol, or phosphatidylserine
674380
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.91
7.4
-
-
Homo sapiens
2.7.1.91
7.4
-
-
Rattus norvegicus