EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | M42A | 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42C | slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42E | 6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42G | 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42H | 40% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42I | slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42L | slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42P | 50% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42Q | 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42S | 45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42T | slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42V | slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42W | 6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
1.5.1.3 | M42Y | 30% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.00061 | - |
7,8-dihydrofolate | mutant M42I, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00082 | - |
7,8-dihydrofolate | mutant M42T, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00083 | - |
7,8-dihydrofolate | mutant M42P, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00091 | - |
7,8-dihydrofolate | mutant M42V, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00112 | - |
7,8-dihydrofolate | mutant M42L, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00118 | - |
7,8-dihydrofolate | mutant M42G, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.0013 | - |
7,8-dihydrofolate | wild-type, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.0013 | - |
7,8-dihydrofolate | mutant M42A, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00178 | - |
7,8-dihydrofolate | mutant M42Y, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00193 | - |
7,8-dihydrofolate | mutant M42H, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00194 | - |
7,8-dihydrofolate | mutant M42C, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00227 | - |
7,8-dihydrofolate | mutant M42Q, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00227 | - |
7,8-dihydrofolate | mutant M42S, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.00597 | - |
7,8-dihydrofolate | mutant M42E, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 0.045 | - |
7,8-dihydrofolate | mutant M42W, pH 7.0, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 12.4 | - |
7,8-dihydrofolate | mutant M42I, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 13 | - |
7,8-dihydrofolate | mutant M42G, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 14 | - |
7,8-dihydrofolate | mutant M42A, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 15.7 | - |
7,8-dihydrofolate | mutant M42V, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 15.9 | - |
7,8-dihydrofolate | mutant M42E, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 18.7 | - |
7,8-dihydrofolate | mutant M42T, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 18.9 | - |
7,8-dihydrofolate | mutant M42L, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 22.6 | - |
7,8-dihydrofolate | mutant M42S, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 24 | - |
7,8-dihydrofolate | mutant M42P, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 24.3 | - |
7,8-dihydrofolate | mutant M42Q, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 24.6 | - |
7,8-dihydrofolate | wild-type, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 26.6 | - |
7,8-dihydrofolate | mutant M42H, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 30.4 | - |
7,8-dihydrofolate | mutant M42C, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 45.5 | - |
7,8-dihydrofolate | mutant M42Y, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 89 | - |
7,8-dihydrofolate | mutant M42W, pH 7.0, 25°C | Escherichia coli | |
1.5.1.3 | 104.6 | - |
7,8-dihydrofolate | mutant M42W, pH 7.0, 25°C | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Escherichia coli |