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Literature summary extracted from

  • Li, F.; Hagemeier, C.H.; Seedorf, H.; Gottschalk, G.; Thauer, R.K.
    Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase (2007), J. Bacteriol., 189, 4299-4304.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.3 expression in Escherichia coli Clostridium kluyveri
2.3.3.16 expression in Escherichia coli Clostridium kluyveri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.3 aerobic conditions 50% inactivation in 24 h by aerobic conditions Clostridium kluyveri
2.3.3.3 EDTA 0.05 mM, complete inhibition, reactivation by addition of Mg2+, Mn2+, or Co2+ Clostridium kluyveri
2.3.3.3 p-chloromercuribenzoate 0.002 mM, inactivation Clostridium kluyveri
2.3.3.16 additional information the activity is not affected by O2, pCMB (0.05 mM), or EDTA (0.05 mM) Clostridium kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.3 0.04
-
 oxaloacetate pH 8.0 Clostridium kluyveri
2.3.3.3 0.05
-
 acetyl-CoA pH 8.0 Clostridium kluyveri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 additional information no Mn2+, Co2+, or Mg2+ dependency Clostridium kluyveri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.3 51000
-
 x * 52100, calculated, x * 51000, SDS-PAGE Clostridium kluyveri
2.3.3.3 52100
-
 x * 52100, calculated, x * 51000, SDS-PAGE Clostridium kluyveri
2.3.3.3 52100
-
 x * 52100, calculated from sequence Clostridium kluyveri
2.3.3.16 51100
-
 x * 51100, calculated from sequence Clostridium kluyveri

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.3 Clostridium kluyveri
-
-
-
2.3.3.3 Clostridium kluyveri
-
 isoform IPMS2
-
2.3.3.3 Desulfovibrio desulfuricans
-
-
-
2.3.3.3 Desulfovibrio vulgaris
-
-
-
2.3.3.13 Clostridium kluyveri
-
-
-
2.3.3.13 Desulfovibrio desulfuricans
-
-
-
2.3.3.13 Desulfovibrio vulgaris
-
-
-
2.3.3.14 Clostridium kluyveri
-
-
-
2.3.3.16 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.3 recombinant enzyme Clostridium kluyveri
2.3.3.16 recombinantz enzyme Clostridium kluyveri

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.3.3 enzyme inhibited by EDTA can be reactivated by Mg2+, Mn2+, Co2+ Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.3 1.3
-
 pH 8.0 Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate
-
 Clostridium kluyveri citrate + CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.3.3 ? x * 52100, calculated, x * 51000, SDS-PAGE Clostridium kluyveri
2.3.3.3 ? x * 52100, calculated from sequence Clostridium kluyveri
2.3.3.16 ? x * 51100, calculated from sequence Clostridium kluyveri

Synonyms

EC Number Synonyms Comment Organism
2.3.3.3 Re-citrate synthase
-
 Clostridium kluyveri
2.3.3.3 Re-citrate synthase
-
 Desulfovibrio desulfuricans
2.3.3.3 Re-citrate synthase
-
 Desulfovibrio vulgaris
2.3.3.13 IPMS
-
 Desulfovibrio desulfuricans
2.3.3.13 IPMS
-
 Desulfovibrio vulgaris
2.3.3.16 Si-citrate synthase
-
 Clostridium kluyveri