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Literature summary extracted from

  • Cho, E.A.; Lee, D.W.; Cha, Y.H.; Lee, S.J.; Jung, H.C.; Pan, J.G.; Pyun, Y.R.
    Characterization of a novel D-lyxose isomerase from Cohnella laevoribosii RI-39 sp. nov (2007), J. Bacteriol., 189, 1655-1663.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.15 overexpression in Escherichia coli Cohnella laeviribosi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.15 22.4
-
 D-Lyxose
-
 Cohnella laeviribosi
5.3.1.15 34
-
 D-mannose
-
 Cohnella laeviribosi
5.3.1.15 121.7
-
 L-ribose
-
 Cohnella laeviribosi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.15 Co2+ activating at 1 mM, D-xylose as substrate Cohnella laeviribosi
5.3.1.15 Mn2+ at 1 mM, D-xylose as substrate, requires divalent metal ions Cohnella laeviribosi
5.3.1.15 Ni2+ activating at 1 mM, D-xylose as substrate Cohnella laeviribosi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.15 42000
-
 gel filtration Cohnella laeviribosi

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.15 Cohnella laeviribosi A3E7Z6
-
-
5.3.1.15 Cohnella laeviribosi RI-39 A3E7Z6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.15 of the native protein to homogeneity by DEAE-Sephacel ion-exchange chromatography, followed by RESOURCE PHE hydrophobic chromatography, of the recombinant protein Cohnella laeviribosi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.15 8.4
-
 of the native enzyme after purification, at 70°C, with L-ribose as substrate Cohnella laeviribosi
5.3.1.15 50.2
-
 of the recombinant enzyme after purification, at 70°C, with L-ribose as substrate Cohnella laeviribosi

Storage Stability

EC Number Storage Stability Organism
5.3.1.15 at -20°C for several months Cohnella laeviribosi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.15 D-Lyxose
-
 Cohnella laeviribosi D-Xylulose
-
 r
5.3.1.15 D-Lyxose
-
 Cohnella laeviribosi RI-39 D-Xylulose
-
 r
5.3.1.15 D-Mannose no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate Cohnella laeviribosi D-Fructose
-
 r
5.3.1.15 D-Mannose no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate Cohnella laeviribosi RI-39 D-Fructose
-
 r
5.3.1.15 L-ribose
-
 Cohnella laeviribosi L-ribulose
-
 r
5.3.1.15 L-ribose
-
 Cohnella laeviribosi RI-39 L-ribulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.15 dimer 2 x 21000 SDS-PAGE Cohnella laeviribosi

Synonyms

EC Number Synonyms Comment Organism
5.3.1.15 CLLI D-lyxose isomerase from Cohnella laevoribosii Cohnella laeviribosi
5.3.1.15 CLRI L-ribose isomerase from Cohnella laevoribosii Cohnella laeviribosi
5.3.1.15 D-Lyxose isomerase
-
 Cohnella laeviribosi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.15 70
-
 wild-type and recombinant enzymes at 1 mM Mn2+ Cohnella laeviribosi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.15 6.5
-
 wild-type and recombinant enzymes at 1 mM Mn2+ Cohnella laeviribosi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.15 Cohnella laeviribosi wild-type and recombinant enzymes
-
 4.2