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Literature summary extracted from

  • Yoshida, M.; Oikawa, T.; Obata, H.; Abe, K.; Mihara, H.; Esaki, N.
    Biochemical and genetic analysis of the gamma-resorcylate (2,6-dihydroxybenzoate), aatabolic pathway in Rhizobium sp. strain MTP-10005: Identification and functional analysis of its gene cluster (2006), J. Bacteriol., 189, 1573-1581.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.1.32 additional information graDAFCBE genes are responsible as an operon for the growth of Rhizobium sp. strain MTP-10005 on gamma-resorcylate and are probably regulated by GraR at the transcriptional level, first report of the gamma-resorcylate catabolic pathway in an aerobic bacterium Rhizobium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 into a pT7Blue T-Vector and expressed in Escherichia coli BL21 Star (DE3) Rhizobium sp.
1.14.14.27 expressed in Escherichia coli Rhizobium sp. MTP-10005

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 36410
-
 predicted Rhizobium sp.
1.14.14.27 45500
-
-
 Rhizobium sp. MTP-10005

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Rhizobium sp. A1IIX4
-
-
1.14.14.27 Rhizobium sp. MTP-10005 A1IIX2
-
-
4.1.1.103 Rhizobium sp. Q60GU1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 13.5
-
-
 Rhizobium sp.

Subunits

EC Number Subunits Comment Organism
1.14.14.27 ? x * 45500, oxygenase components of resorcinol hydroxylase, SDS-PAGE Rhizobium sp. MTP-10005

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 GraC
-
 Rhizobium sp.
1.14.14.27 graA
-
 Rhizobium sp. MTP-10005
4.1.1.103 graF
-
 Rhizobium sp.

Expression

EC Number Organism Comment Expression
4.1.1.103 Rhizobium sp. the maximum relative mRNA expression level of graF is detected after one h of cultivation when gamma-resorcylate is used as the sole carbon source. The graDAFCBE genes are transcribed as a single mRNA and the transcription of the gene cluster is induced by gamma-resorcylate up