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Literature summary extracted from

  • Dhiman, R.K.; Schaeffer, M.L.; Bailey, A.M.; Testa, C.A.; Scherman, H.; Crick, D.C.
    1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin (2005), J. Bacteriol., 187, 8395-8402.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene ispC, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), Rv2870c complements an inactivated chromosomal copy of IspC in Salmonella enterica, and the complemented strain is sensitive to fosmidomycin Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 1-deoxy-D-xylulose 5-phosphate substrate inhibition Mycobacterium tuberculosis
1.1.1.267 EDTA complete inhibition at 5 mM Mycobacterium tuberculosis
1.1.1.267 fosmidomycin IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteria and other organisms Mycobacterium tuberculosis
1.1.1.267 additional information no substrate inhibition by 2-C-methyl-D-erythritol 4-phosphate, NADPH or NADP+ Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0297
-
 NADPH pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
1.1.1.267 0.0471
-
 1-deoxy-D-xylulose 5-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
1.1.1.267 0.174
-
 2-C-methyl-D-erythritol 4-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
1.1.1.267 0.561
-
 NADP+ pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 cytosol
-
 Mycobacterium tuberculosis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ absolutely dependent on, optimal at 4 mM Mycobacterium tuberculosis
1.1.1.267 additional information Mn2+, Ca2+, Co2+, Zn2+, and Fe2+ ions are unable to support activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.267 45000
-
 x * 45000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis H37Rv first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Mycobacterium tuberculosis
-
 gene ispC
-
1.1.1.267 Mycobacterium tuberculosis H37Rv
-
 gene ispC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.267 ? x * 45000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 IspC
-
 Mycobacterium tuberculosis
1.1.1.267 Rv2870c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 30
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5 7.9 forward and reverse reaction Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.267 5 9
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
-
 Mycobacterium tuberculosis
1.1.1.267 NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 1.1
-
 1-deoxy-D-xylulose 5-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 0.00031
-
 IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri Mycobacterium tuberculosis fosmidomycin
1.1.1.267 0.0027
-
 IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri Mycobacterium tuberculosis fosmidomycin