BRENDA - Enzyme Database

Glutamyl-tRNA reductase of Chlorobium vibrioforme is a dissociable homodimer that contains one tightly bound heme per subunit

Srivastava, A.; Beale, S.I.; J. Bacteriol. 187, 4444-4450 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.70
glutamate-1-semialdehyde aminotransferase
2.5fold activity in presence of glutamate-1-semialdehyde aminotransferase
Prosthecochloris vibrioformis
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
expression in Escherichia coli
Prosthecochloris vibrioformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
49000
-
SDS-PAGE, recombinant protein
Prosthecochloris vibrioformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Prosthecochloris vibrioformis
P28462
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
Ni-affinity chromatography
Prosthecochloris vibrioformis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.70
cell culture
-
Prosthecochloris vibrioformis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADH + H+
-
674242
Prosthecochloris vibrioformis
L-glutamate 1-semialdehyde + NAD+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
dimer
2 * 49000, gel-filtration in presence of 5% glycerol
Prosthecochloris vibrioformis
1.2.1.70
monomer
1 * 49000, gel-filtration without glycerol
Prosthecochloris vibrioformis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
one molecule per glutamyl-tRNA reductase molecule
Prosthecochloris vibrioformis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.70
glutamate-1-semialdehyde aminotransferase
2.5fold activity in presence of glutamate-1-semialdehyde aminotransferase
Prosthecochloris vibrioformis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
expression in Escherichia coli
Prosthecochloris vibrioformis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
one molecule per glutamyl-tRNA reductase molecule
Prosthecochloris vibrioformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
49000
-
SDS-PAGE, recombinant protein
Prosthecochloris vibrioformis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
Ni-affinity chromatography
Prosthecochloris vibrioformis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.70
cell culture
-
Prosthecochloris vibrioformis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADH + H+
-
674242
Prosthecochloris vibrioformis
L-glutamate 1-semialdehyde + NAD+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
dimer
2 * 49000, gel-filtration in presence of 5% glycerol
Prosthecochloris vibrioformis
1.2.1.70
monomer
1 * 49000, gel-filtration without glycerol
Prosthecochloris vibrioformis