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Literature summary extracted from

  • Wang, P.F.; Kenyon, G.L.; McLeish, M.J.
    Heterogeneity of Escherichia coli-expressed human muscle creatine kinase (2006), IUBMB Life, 58, 421-428.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 additional information expression of enzyme in Escherichia coli results in four different isoforms having similar kinetic parameters and identical bands on SDS-PAGE but different anodal mobility on non-denaturing gels. Cause of isoform formation may be deamidation of asparagine or glutamine residues Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.34
-
 ATP isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 0.36
-
 ATP isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 0.4
-
 ATP wild-type and isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 0.43
-
 ATP isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 2 3 Creatine isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 19
-
 Creatine isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 20
-
 Creatine isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 21
-
 Creatine wild-type and isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Homo sapiens
-
 expression in Escherichia coli
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Homo sapiens ADP + creatine phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.2 76
-
 ATP isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 88
-
 ATP isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 115
-
 ATP isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens
2.7.3.2 143
-
 ATP wild-type, pH 8.0, 30°C Homo sapiens
2.7.3.2 155
-
 ATP isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C Homo sapiens