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Literature summary extracted from

  • Feng, S.; Zhao, T.J.; Zhou, H.M.; Yan, Y.B.
    Effects of the single point genetic mutation D54G on muscle creatine kinase activity, structure and stability (2007), Int. J. Biochem. Cell Biol., 39, 392-401.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 D54G mutation identified in acute myocardial infarction patient, mutant shows substantially decreased activity, substrate binding affinity and stability Homo sapiens
2.7.3.2 D54G the mutant enzyme has substantially decreased activity, substrate binding affinity and stability. Spectroscopic experiments indicate that the mutation impairs the structure of creatine kinase, which results in a partially unfolded state with more hydrophobic exposure and exposed Trp residues. The inability to fold to the functional compact state makes the mutant be prone to aggregate upon microenvironmental stresses, and might gradually decrease the creatine kinase level of the acute myocardial infarction patient Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.46
-
 ATP wild-type enzyme Homo sapiens
2.7.3.2 0.46
-
 ATP wild-type Homo sapiens
2.7.3.2 2.2
-
 ATP mutant D54G Homo sapiens
2.7.3.2 2.2
-
 ATP mutant enzyme D54G Homo sapiens
2.7.3.2 9.6
-
 Creatine wild-type enzyme Homo sapiens
2.7.3.2 9.6
-
 Creatine wild-type Homo sapiens
2.7.3.2 34
-
 Creatine mutant D54G Homo sapiens
2.7.3.2 34
-
 Creatine mutant enzyme D54G Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Homo sapiens
-
 acute myocardial infarction patient
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 52
-
 mutant D54G Homo sapiens
2.7.3.2 52
-
 mutant enzyme D54G Homo sapiens
2.7.3.2 245.5
-
 wild-type enzyme Homo sapiens
2.7.3.2 246
-
 wild-type Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Homo sapiens ADP + phosphocreatine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.2 36
-
 mutant D54G, melting temperature Homo sapiens
2.7.3.2 37
-
 midpoint temperature of thermal inactivation of mutant enzyme D54G is 36.5°C Homo sapiens
2.7.3.2 45
-
 10 min, mutant enzyme D54G is completely inactivated Homo sapiens
2.7.3.2 48
-
 wild-type, stable up to 48°C Homo sapiens
2.7.3.2 48
-
 10 min, the activity of wild-type enzyme has no significant changes after heat treatment at temperatures below 48°C Homo sapiens
2.7.3.2 52
-
 wild-type, melting temperature Homo sapiens
2.7.3.2 53
-
 midpoint temperature of thermal inactivation of wild-type enzyme is 52.5°C Homo sapiens
2.7.3.2 58
-
 wild-type, complete loss of activity Homo sapiens
2.7.3.2 58
-
 10 min, complete loss of activity above Homo sapiens