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Literature summary extracted from

  • Lee, Y.Y.; Westphal, A.H.; de Haan, L.H.; Aarts, J.M.; Rietjens, I.M.; van Berkel, W.J.
    Human NAD(P)H:quinone oxidoreductase inhibition by flavonoids in living cells (2005), Free Radic. Biol. Med., 39, 257-265.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.6.5.2 analysis method to measure enzyme inhibition in intact cells based on the resorufin reductase activity of enzyme. Values for 50% inhibition of enzyme by several reported in vivo inhibitors is at least three orders of magnitude higher for intact cells than for cell lysates Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 7,8-dihydroxyflavone
-
 Homo sapiens
1.6.5.2 7-hydroxyflavone
-
 Homo sapiens
1.6.5.2 chrysin
-
 Homo sapiens
1.6.5.2 dicoumarol
-
 Homo sapiens
1.6.5.2 isorhamnetin
-
 Homo sapiens
1.6.5.2 quercetin
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Homo sapiens
-
 recombinant enzyme expressed in Chinese hamster ovary cells
-

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.2 0.0005
-
 dicoumarol value for lysed cells Homo sapiens
1.6.5.2 0.016
-
 7,8-dihydroxyflavone value for lysed cells Homo sapiens
1.6.5.2 0.017
-
 chrysin value for lysed cells Homo sapiens
1.6.5.2 0.309
-
 7-hydroxyflavone value for lysed cells Homo sapiens
1.6.5.2 1.03
-
 isorhamnetin value for lysed cells Homo sapiens
1.6.5.2 6.19
-
 quercetin value for lysed cells Homo sapiens