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Literature summary extracted from

  • Reher, M.; Bott, M.; Schoenheit, P.
    Characterization of glycerate kinase (2-phosphoglycerate forming), a key enzyme of the nonphosphorylative Entner-Doudoroff pathway, from the thermoacidophilic euryarchaeon Picrophilus torridus (2006), FEMS Microbiol. Lett., 259, 113-119.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.34
-
 (R)-glycerate
-
 Picrophilus torridus
2.7.1.165 0.5
-
 ATP
-
 Picrophilus torridus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Mg2+ 0.05 mM, most effective divalent cation Picrophilus torridus
2.7.1.165 additional information Mg2+ can be replaced to some extent by Ni2+ (25%), Mn2+ (11%), and Co2+ (11%) Picrophilus torridus
2.7.1.165 additional information no activating effect of K+ Picrophilus torridus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 47500
-
 2 * 47500, gel filtration Picrophilus torridus
2.7.1.165 50000
-
 SDS-PAGE, denatured enzyme Picrophilus torridus
2.7.1.165 93000
-
 calculated from amino acid sequence Picrophilus torridus
2.7.1.165 95000
-
 gel filtration, native enzyme Picrophilus torridus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Picrophilus torridus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165 Resource Phenyl column chromatography, Superdex 200 HiLoad 16/60 column gel filtration, and UNO-Q1 column chromatography Picrophilus torridus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.165 0.07
-
 cell extract, at 50°C Picrophilus torridus
2.7.1.165 358
-
 after 5114fold purification, at 50°C Picrophilus torridus

Storage Stability

EC Number Storage Stability Organism
2.7.1.165 60°C, 100 mM Tris-HCl at pH 8.0, 2 h, no loss of activity Picrophilus torridus
2.7.1.165 70°C, 100 mM Tris-HCl at pH 8.0, 5 min, 50% loss of activity Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate
-
 Picrophilus torridus ADP + 3-phospho-(R)-glycerate
-
 ?
2.7.1.165 additional information the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate Picrophilus torridus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 homodimer 2 * 47500, gel filtration Picrophilus torridus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.165 GCK
-
 Picrophilus torridus
2.7.1.165 glycerate kinase (2-phosphoglycerate forming)
-
 Picrophilus torridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 60
-
-
 Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.165 7.3
-
-
 Picrophilus torridus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.165 5.6 8.6 50% of activity at pH 5.6 and at pH 8.6 Picrophilus torridus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.165 ATP
-
 Picrophilus torridus