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Literature summary extracted from

  • Zhao, T.J.; Feng, S.; Wang, Y.L.; Liu, Y.; Luo, X.C.; Zhou, H.M.; Yan, Y.B.
    Impact of intra-subunit domain-domain interactions on creatine kinase activity and stability (2006), FEBS Lett., 580, 3835-3840.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 A76G mutation in the intra-subunit domain-domain interface, similar to wild-type in kinetics and thermal inactivation Oryctolagus cuniculus
2.7.3.2 C74A mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation Oryctolagus cuniculus
2.7.3.2 C74L mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation Oryctolagus cuniculus
2.7.3.2 C74M mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation Oryctolagus cuniculus
2.7.3.2 C74S mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation Oryctolagus cuniculus
2.7.3.2 G73A mutation in the intra-subunit domain-domain interface, decrease in activity and stability Oryctolagus cuniculus
2.7.3.2 V72A mutation in the intra-subunit domain-domain interface, decrease in activity and stability Oryctolagus cuniculus
2.7.3.2 V75A mutation in the intra-subunit domain-domain interface, no significant effect on activity and structure, decrease in stability and reactivation Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.43
-
 ATP mutant C74A, 25°C Oryctolagus cuniculus
2.7.3.2 0.45
-
 ATP wild-type, 25°C Oryctolagus cuniculus
2.7.3.2 0.46
-
 ATP mutant A76G, 25°C Oryctolagus cuniculus
2.7.3.2 0.47
-
 ATP mutant C74S, 25°C Oryctolagus cuniculus
2.7.3.2 0.51
-
 ATP mutant V72A, 25°C Oryctolagus cuniculus
2.7.3.2 0.57
-
 ATP mutant V75A, 25°C Oryctolagus cuniculus
2.7.3.2 0.66
-
 ATP mutant C74M, 25°C Oryctolagus cuniculus
2.7.3.2 0.68
-
 ATP mutant G73A, 25°C Oryctolagus cuniculus
2.7.3.2 0.7
-
 ATP mutant C74L, 25°C Oryctolagus cuniculus
2.7.3.2 8.7
-
 Creatine mutant C74A, 25°C Oryctolagus cuniculus
2.7.3.2 8.9
-
 Creatine mutant A76G, 25°C Oryctolagus cuniculus
2.7.3.2 9
-
 Creatine mutant C74S, 25°C Oryctolagus cuniculus
2.7.3.2 9.1
-
 Creatine wild-type, 25°C Oryctolagus cuniculus
2.7.3.2 13.8
-
 Creatine mutant V75A, 25°C Oryctolagus cuniculus
2.7.3.2 18.3
-
 Creatine mutant C74M, 25°C Oryctolagus cuniculus
2.7.3.2 18.6
-
 Creatine mutant C74L, 25°C Oryctolagus cuniculus
2.7.3.2 21.4
-
 Creatine mutant V72A, 25°C Oryctolagus cuniculus
2.7.3.2 25
-
 Creatine mutant G73A, 25°C Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Oryctolagus cuniculus P00563
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 148
-
 mutant V72A, 25°C Oryctolagus cuniculus
2.7.3.2 256
-
 mutant C74M, 25°C Oryctolagus cuniculus
2.7.3.2 258
-
 mutant C74L, 25°C Oryctolagus cuniculus
2.7.3.2 261
-
 wild-type, 25°C Oryctolagus cuniculus
2.7.3.2 263
-
 mutant V75A, 25°C Oryctolagus cuniculus
2.7.3.2 266
-
 mutant C74S, 25°C Oryctolagus cuniculus
2.7.3.2 272
-
 mutant A76G, 25°C Oryctolagus cuniculus
2.7.3.2 278
-
 mutant C74A, 25°C Oryctolagus cuniculus
2.7.3.2 8208
-
 mutant G73A, 25°C Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Oryctolagus cuniculus ADP + phosphocreatine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.2 45
-
 mutant G73A, inactivation Oryctolagus cuniculus
2.7.3.2 48
-
 wild-type, stable up to 48°C, mutant V75A, inactivation Oryctolagus cuniculus
2.7.3.2 58
-
 wild-type, inactivation Oryctolagus cuniculus