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Literature summary extracted from

  • Sayanova, O.; Haslam, R.; Qi, B.; Lazarus, C.M.; Napier, J.A.
    The alternative pathway C20 DELTA8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase (2006), FEBS Lett., 580, 1946-1952.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4 cloned into pBINI19-35S, transferred to Agrobacterium tumefaciens strain GV3101 and expressed in wild-type Arabidopsis Col 0 or in transgenic line CA1-9 expressing the Isochrysis galbana elongating activity IgASE1 Acanthamoeba castellanii
1.14.19.4 cloned into the yeast expression vector pYES2 and expressed in Saccharomyces serevisiae strain W303-1A Acanthamoeba castellanii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.4 A44G expressed in yeast, 2-fold higher activity toward 20:2 (n-6), but nor alternation in activity towards 20:3 (n-3) in comparison with the wild-type Acanthamoeba castellanii

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Acanthamoeba castellanii
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Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8 desaturase
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 Acanthamoeba castellanii