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Literature summary extracted from

  • Topalis, D.; Kumamoto, H.; Amaya Velasco, M.F.; Dugue, L.; Haouz, A.; Alexandre, J.A.; Gallois-Montbrun, S.; Alzari, P.M.; Pochet, S.; Agrofoglio, L.A.; Deville-Bonne, D.
    Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives - a screening based on affinity for the UMP-CMP binding site (2007), FEBS J., 274, 3704-3714.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.4.14 medicine screening of acyclic phosphonate analogs to find antimetabolites for antivirus and anticancer therapies Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.14 overexpression in Escherichia coli, wild type and selenomethionine labelled protein Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.14 of the selenomethionine labelled protein in complex with UMP, CMP, ADP, AMP-PCP, cidofovor and P1-(5’-adenosyl) P5-(5’-uridyl) pentaphosphate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 5-Br-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 5-Cl-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 5-F-Phe-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 5-Phe-S-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 5-Phe-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 uracil vinylphosphonate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.07
-
 CMP
-
 Homo sapiens
2.7.4.14 0.13
-
 UMP
-
 Homo sapiens
2.7.4.14 1
-
 Cidofovir
-
 Homo sapiens
2.7.4.14 1.1
-
 dCMP
-
 Homo sapiens
2.7.4.14 1.3
-
 dUMP
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Homo sapiens P30085
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14 of the recombinant protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine Cidofovir Homo sapiens ADP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
-
 ?
2.7.4.14 ATP + CMP
-
 Homo sapiens ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP
-
 Homo sapiens ADP + dCDP
-
 ?
2.7.4.14 ATP + dUMP no activity with CDP, dTMP, AMP, GMP Homo sapiens ADP + dUDP
-
 ?
2.7.4.14 ATP + UMP
-
 Homo sapiens ADP + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.14 cytidylate kinase
-
 Homo sapiens
2.7.4.14 UCK
-
 Homo sapiens
2.7.4.14 UMP-CMP kinase
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.14 0.016
-
 5-Cl-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 0.017
-
 5-Br-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 0.028
-
 5-Phe-S-uracil vinylphosphonate
-
 Homo sapiens
2.7.4.14 0.7
-
 uracil vinylphosphonate
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.14 0.03
-
-
 Homo sapiens 5-Cl-uracil vinylphosphonate
2.7.4.14 0.035
-
-
 Homo sapiens 5-Br-uracil vinylphosphonate
2.7.4.14 0.05
-
-
 Homo sapiens 5-Phe-S-uracil vinylphosphonate
2.7.4.14 0.12
-
 amplitude 50% Homo sapiens 5-Phe-S-uracil vinylphosphonate
2.7.4.14 0.23
-
-
 Homo sapiens 5-F-Phe-uracil vinylphosphonate
2.7.4.14 1.2
-
-
 Homo sapiens uracil vinylphosphonate