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Literature summary extracted from
- Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with gamma-cyclodextrin (2007), FEBS J., 274, 1001-1010.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.19 | more than 80% of the initial immobilized and crosslinked imprinted CGTase activity is retained for up to five cycles of synthesis reactions | Paenibacillus macerans |
| 2.4.1.19 | more than 80% of the initial immobilized and crosslinked imprinted CGTase activity is retained for up to five cycles of synthesis reactions | Paenibacillus sp. |
| 2.4.1.19 | the immobilized and CD8-crosslinked imprinted CGTase shows 15% higher stability in phosphate buffer containing up to 50% ethanol or cyclohexane compared to the native enzyme | Paenibacillus macerans |
| 2.4.1.19 | the immobilized and CD8-crosslinked imprinted CGTase shows 30% higher stability in phosphate buffer containing up to 50% ethanol or cyclohexane compared to the native enzyme | Paenibacillus sp. |
| 2.4.1.19 | the temperature stability of the immobilized and crosslinked imprinted CGTase at 60°C is considerably higher than that of the native enzyme | Paenibacillus macerans |
| 2.4.1.19 | the temperature stability of the immobilized and crosslinked imprinted CGTase at 60°C is considerably higher than that of the native enzyme | Paenibacillus sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.19 | additional information | - |
additional information | - |
Paenibacillus macerans | |
| 2.4.1.19 | additional information | - |
additional information | - |
Paenibacillus sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.19 | Paenibacillus macerans | - |
- |
- |
| 2.4.1.19 | Paenibacillus sp. | - |
- |
- |
| 2.4.1.19 | Paenibacillus sp. A11 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | starch | to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 15:65:20:0 at 40°C. The size of the synthesis products formed by the crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes | Paenibacillus sp. | cyclodextrin | - |
? |  |
| 2.4.1.19 | starch | to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 43:36:21:0 at 40°C. The size of the synthesis products formed bythe crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes | Paenibacillus macerans | cyclodextrin | - |
? | |
| 2.4.1.19 | starch | to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 15:65:20:0 at 40°C. The size of the synthesis products formed by the crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes | Paenibacillus sp. A11 | cyclodextrin | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 40 | 50 | native enzyme | Paenibacillus sp. |
| 2.4.1.19 | 60 | - |
native enzyme | Paenibacillus macerans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 30 | 70 | 30°C: about 45% of maximal activity, 70°C: about 80% of maximal activity, native enzyme | Paenibacillus macerans |
| 2.4.1.19 | 30 | 70 | 30°C: about 450% of maximal activity, 90°C: about 50% of maximal activity, native enzyme | Paenibacillus sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 60 | - |
pH 6.0, 30 min, native enzyme shows about 10% loss of activity. Iimmobilized and crosslinked imprinted CGTase are stable | Paenibacillus sp. |
| 2.4.1.19 | 60 | - |
pH 6.0, 30 min, native enzyme shows about 10% loss of activity. Immobilized and crosslinked imprinted CGTase are stable | Paenibacillus macerans |
| 2.4.1.19 | 70 | - |
pH 6.0, 30 min, native enzyme is completely inactivated. Immobilized and crosslinked imprinted CGTase shows about 50% loss of activity | Paenibacillus sp. |
| 2.4.1.19 | 70 | - |
pH 6.0, 30 min, native enzyme shows about 50% loss of activity. Immobilized and crosslinked imprinted CGTase show about 15% loss of activity | Paenibacillus macerans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 6 | - |
native enzyme | Paenibacillus macerans |
| 2.4.1.19 | 6 | 8 | native enzyme | Paenibacillus sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 5 | 9 | pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity, native enzyme | Paenibacillus sp. |
| 2.4.1.19 | 5 | 7 | pH 5.0: about 70% of maximal activity, pH 9.0: about 70% of maximal activity, native enzyme | Paenibacillus macerans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | additional information | - |
the immobilized and the crosslinked imprinted CGTase are more stable than the native enzyme in the pH ranges from 3 to 6 and 8 to 11 | Paenibacillus sp. |
| 2.4.1.19 | additional information | - |
the immobilized and the crosslinked imprinted CGTase shows higher stability in the ranges from pH 3 to 7 and from pH 9 to 11 | Paenibacillus macerans |
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