EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Halalkalibacterium halodurans |
1.5.1.3 | expression in Escherichia coli | Halalkalibacterium halodurans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | additional information | alkaliphilic properties of enzyme are diminished upon addition of a N-terminal His-tag | Halalkalibacterium halodurans |
1.5.1.3 | additional information | a C-terminal His-tag does not affect the catalytic properties of the enzyme, but results in loss of the thermal stability and of the alkali-stable phenotype | Halalkalibacterium halodurans |
EC Number | General Stability | Organism |
---|---|---|
1.5.1.3 | 50% residual activity in 4 M urea | Halalkalibacterium halodurans |
1.5.1.3 | stable in NaCl or KCl up to 0.3 M with slight activation below 0.1 M | Halalkalibacterium halodurans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | methotrexate | wild-type, 50% inhibition at 0.00031 mM | Halalkalibacterium halodurans | |
1.5.1.3 | additional information | stable in up to 3 M urea; wild-type enzyme is not affected by presence of NaCl or KCl up to a concentration of 0.3 M, while the His-tagged enzyme is affected even at low concentrations | Halalkalibacterium halodurans | |
1.5.1.3 | trimethoprim | wild-type, 50% inhibition at 0.0089 mM | Halalkalibacterium halodurans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0013 | - |
7,8-dihydrofolate | wild-type, pH 7.5, 37°C | Halalkalibacterium halodurans | |
1.5.1.3 | 0.0029 | - |
7,8-dihydrofolate | His-tagged recombinant enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | |
1.5.1.3 | 0.0029 | - |
7,8-dihydrofolate | His-tagged enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | |
1.5.1.3 | 0.0059 | - |
NADPH | His-tagged recombinant enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | |
1.5.1.3 | 0.0059 | - |
NADPH | His-tagged enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | |
1.5.1.3 | 0.0082 | - |
NADPH | wild-type, pH 7.5, 37°C | Halalkalibacterium halodurans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 18855 | - |
1 * 18855, mass spectrometry, dynamic light scattering | Halalkalibacterium halodurans |
1.5.1.3 | 20157 | - |
x + 18855, wild-type, x * 20157, His-tagged enzyme, mass spectrometry | Halalkalibacterium halodurans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Halalkalibacterium halodurans | - |
- |
- |
1.5.1.3 | Halalkalibacterium halodurans | - |
expression in Escherichia coli | - |
1.5.1.3 | Halalkalibacterium halodurans C-125 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 122.9 | - |
pH 7.5, 37°C | Halalkalibacterium halodurans |
1.5.1.3 | 122.9 | - |
His-tagged enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans |
1.5.1.3 | 138.4 | - |
wild-type, pH 7.5, 37°C | Halalkalibacterium halodurans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Halalkalibacterium halodurans | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Halalkalibacterium halodurans C-125 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Halalkalibacterium halodurans | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Halalkalibacterium halodurans C-125 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | ? | x + 18855, wild-type, x * 20157, His-tagged enzyme, mass spectrometry | Halalkalibacterium halodurans |
1.5.1.3 | monomer | 1 * 18855, mass spectrometry, dynamic light scattering | Halalkalibacterium halodurans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 45 | - |
His-tagged enzyme, stable up to | Halalkalibacterium halodurans |
1.5.1.3 | 45 | - |
stable up to, His-tagged enzyme | Halalkalibacterium halodurans |
1.5.1.3 | 50 | - |
wild-type, stable for 3 h | Halalkalibacterium halodurans |
1.5.1.3 | 50 | - |
3 h, stable, wild-type | Halalkalibacterium halodurans |
1.5.1.3 | 52.5 | - |
wild-type, halflife of 60 min, His-tagged recombinant enzyme, halflife 15 min | Halalkalibacterium halodurans |
1.5.1.3 | 52.5 | - |
half-life 60 min for wild-type, 15 min for His-tagged enzyme, respectively | Halalkalibacterium halodurans |
1.5.1.3 | 60 | - |
wild-type, complete inactivation above | Halalkalibacterium halodurans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 6.75 | 7.75 | His-tagged recombinant enzyme | Halalkalibacterium halodurans |
1.5.1.3 | 6.75 | 7.5 | His-tagged enzyme | Halalkalibacterium halodurans |
1.5.1.3 | 7.25 | 8.75 | wild-type | Halalkalibacterium halodurans |
1.5.1.3 | 7.25 | 8.25 | broad, wild-type | Halalkalibacterium halodurans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 9 | - |
rapid decrease in activity above, wild-type | Halalkalibacterium halodurans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 9 | - |
rapid decrease in stability above | Halalkalibacterium halodurans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADP+ | - |
Halalkalibacterium halodurans | |
1.5.1.3 | NADPH | - |
Halalkalibacterium halodurans |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.00025 | - |
His-tagged enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | methotrexate | |
1.5.1.3 | 0.00031 | - |
wild-type, pH 7.5, 37°C | Halalkalibacterium halodurans | methotrexate | |
1.5.1.3 | 0.0089 | - |
wild-type, pH 7.5, 37°C | Halalkalibacterium halodurans | trimethoprim | |
1.5.1.3 | 0.0101 | - |
His-tagged enzyme, pH 7.5, 37°C | Halalkalibacterium halodurans | trimethoprim |